Isolation and characterization of a starchless mutant of Arabidopsis thaliana (L. ) Heynh lacking ADPglucose pyrophosphorylase activity
- Michigan State Univ., East Lansing (USA)
A mutant of Arabidopsis thaliana lacking ADPglucose pyrophosphorylase activity (EC 2.7.7.27) was isolated (from a mutagenized population of plants) by screening for the absence of leaf starch. The mutant grows as vigorously as the wild type in continuous light but more slowly than the wild type in a 12 hours light/12 hours dark photoperiod. Genetic analysis showed that the deficiency of both starch and ADPglucose pyrophosphorylase activity were attributable to a single, nuclear, recessive mutation at a locus designated adg1. The absence of starch in the mutant demonstrates that starch synthesis in the chloroplast is entirely dependent on a pathway involving ADPglucose pyrophosphorylase. Analysis of leaf extracts by two-dimensional polyacrylamide gel electrophoresis followed by Western blotting experiments using antibodies specific for spinach ADPglucose pyrophosphorylase showed that two proteins, present in the wild type, were absent from the mutant. The heterozygous F{sub 1} progeny of a cross between the mutant and wild type had a specific activity of ADP-glucose pyrophosphorylase indistinguishable from the wild type. These observations suggest that the mutation in the adg1 gene in TL25 might affect a regulatory locus.
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 7025896
- Journal Information:
- Plant Physiology; (USA), Vol. 86:4; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARABIDOPSIS
GENE REGULATION
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
ANTIBODIES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CHLOROPLASTS
ELECTROPHORESIS
GENE MUTATIONS
LEAVES
MUTANTS
PHOTOPERIOD
STARCH
CARBOHYDRATES
CELL CONSTITUENTS
ENZYMES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MUTATIONS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYSACCHARIDES
REAGENTS
SACCHARIDES
SYNTHESIS
TRANSFERASES
551000* - Physiological Systems