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Title: Oligogalacturonides induce flowers in tobacco thin cell layers

Abstract

An optimized tobacco thin-cell-layer (TCL) bioassay was used to study the induction of flowers by plant oligosaccharins. Endopolygalacturonase (EPG)-released fragments of suspension-cultured sycamore cell walls induced flowers on TCLs grown on a medium containing 1.5 {mu}M IBA and 0.9 {mu}M kinetin. The EPG-released fragments were primarily composed of the polysaccharides rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II), and {alpha}-1,4-linked oligogalacturonides. The {alpha}-1,4-linked oligogalacturonides, subsequently purified from the EPG-released sycamore cell wall fragment mixture, induced flowers on TCLs. Purified RG-I and RG-II did not induce flowers. Oligosaccharide fragments, generated by partial acid hydrolysis of citrus pectin, were also capable of inducing flowers on the TCLs. The active components in the pectin fragment mixture were {alpha}-1,4-linked oligogalacturonides. Oligogalacturonides with a degree of polymerization (DP) of 8-16, at concentrations of {approx} 0.1 {mu}M, induced flowers, while oligogalacturonides with a DP 2-7, even at higher concentrations, did not. Oligogalacturonides have previously been shown to induce the synthesis of phytoalexins, protease inhibitors, lignin, and ethylene in other plant systems. Thus, the ability of {alpha}-1,4-linked oligogalacturonides to induce flower formation in the tobacco TCLs represents a new biological activity of these oligosaccharins.

Authors:
; ; ; ;  [1]
  1. Univ. of Georgia, Athens (USA)
Publication Date:
OSTI Identifier:
7025878
Resource Type:
Journal Article
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 89:4; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; OLIGOSACCHARIDES; BIOLOGICAL FUNCTIONS; PLANT CELLS; PHYSIOLOGY; TOBACCO; CARBOHYDRATES; FUNCTIONS; ORGANIC COMPOUNDS; SACCHARIDES; 551000* - Physiological Systems

Citation Formats

Marfa-Riera, V, Gollin, D, Mohnen, D, Darvill, A, and Albersheim, P. Oligogalacturonides induce flowers in tobacco thin cell layers. United States: N. p., 1989. Web.
Marfa-Riera, V, Gollin, D, Mohnen, D, Darvill, A, & Albersheim, P. Oligogalacturonides induce flowers in tobacco thin cell layers. United States.
Marfa-Riera, V, Gollin, D, Mohnen, D, Darvill, A, and Albersheim, P. Sat . "Oligogalacturonides induce flowers in tobacco thin cell layers". United States.
@article{osti_7025878,
title = {Oligogalacturonides induce flowers in tobacco thin cell layers},
author = {Marfa-Riera, V and Gollin, D and Mohnen, D and Darvill, A and Albersheim, P},
abstractNote = {An optimized tobacco thin-cell-layer (TCL) bioassay was used to study the induction of flowers by plant oligosaccharins. Endopolygalacturonase (EPG)-released fragments of suspension-cultured sycamore cell walls induced flowers on TCLs grown on a medium containing 1.5 {mu}M IBA and 0.9 {mu}M kinetin. The EPG-released fragments were primarily composed of the polysaccharides rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II), and {alpha}-1,4-linked oligogalacturonides. The {alpha}-1,4-linked oligogalacturonides, subsequently purified from the EPG-released sycamore cell wall fragment mixture, induced flowers on TCLs. Purified RG-I and RG-II did not induce flowers. Oligosaccharide fragments, generated by partial acid hydrolysis of citrus pectin, were also capable of inducing flowers on the TCLs. The active components in the pectin fragment mixture were {alpha}-1,4-linked oligogalacturonides. Oligogalacturonides with a degree of polymerization (DP) of 8-16, at concentrations of {approx} 0.1 {mu}M, induced flowers, while oligogalacturonides with a DP 2-7, even at higher concentrations, did not. Oligogalacturonides have previously been shown to induce the synthesis of phytoalexins, protease inhibitors, lignin, and ethylene in other plant systems. Thus, the ability of {alpha}-1,4-linked oligogalacturonides to induce flower formation in the tobacco TCLs represents a new biological activity of these oligosaccharins.},
doi = {},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 89:4,
place = {United States},
year = {1989},
month = {4}
}