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Title: Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina

Abstract

The roots of the temperate seagrass Zostera marina undergo daily periods of anaerobiosis at night. These diurnal periods of anoxia alter many metabolic processes in the roots including carbon and nitrogen metabolism, amino acid synthesis, and synthesis and levels of ATP, ADP and AMP. To further characterize the effects of anaerobiosis, we determined in vivo rates of protein synthesis by measuring the relative incorporation of {sup 35}S-MET in TCA precipitated protein samples. Results from these studies show that in vivo protein synthesis decreases continuously during 12 h of anaerobiosis and correlates with changes in ATP levels under similar conditions. Furthermore, polypeptide patterns obtained by SDS-PAGE and 2D-SDSPAGE indicate that anaerobiosis leads to differential protein synthesis in the roots.

Authors:
;  [1]
  1. (Univ. of Chicago, IL (USA))
Publication Date:
OSTI Identifier:
7025796
Resource Type:
Journal Article
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 89:4; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AQUATIC ORGANISMS; PHYSIOLOGY; PROTEINS; BIOSYNTHESIS; ANOXIA; METHIONINE; SULFUR 35; SULFUR COMPOUNDS; TRACER TECHNIQUES; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIOISOTOPES; SULFUR ISOTOPES; SYNTHESIS; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Smith, R.D., and Alberte, R.S. Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina. United States: N. p., 1989. Web.
Smith, R.D., & Alberte, R.S. Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina. United States.
Smith, R.D., and Alberte, R.S. Sat . "Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina". United States.
@article{osti_7025796,
title = {Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina},
author = {Smith, R.D. and Alberte, R.S.},
abstractNote = {The roots of the temperate seagrass Zostera marina undergo daily periods of anaerobiosis at night. These diurnal periods of anoxia alter many metabolic processes in the roots including carbon and nitrogen metabolism, amino acid synthesis, and synthesis and levels of ATP, ADP and AMP. To further characterize the effects of anaerobiosis, we determined in vivo rates of protein synthesis by measuring the relative incorporation of {sup 35}S-MET in TCA precipitated protein samples. Results from these studies show that in vivo protein synthesis decreases continuously during 12 h of anaerobiosis and correlates with changes in ATP levels under similar conditions. Furthermore, polypeptide patterns obtained by SDS-PAGE and 2D-SDSPAGE indicate that anaerobiosis leads to differential protein synthesis in the roots.},
doi = {},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 89:4,
place = {United States},
year = {1989},
month = {4}
}