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Title: /sup 31/P NMR characterization of graded traumatic brain injury in rats

Abstract

Irreversible tissue injury following central nervous system trauma is believed to result from both mechanical disruption at the time of primary insult, and more delayed autodestructive processes. These delayed events are associated with various biochemical changes, including alterations in phosphate energy metabolism and intracellular pH. Using /sup 31/P NMR, we have monitored the changes in phosphorus energy metabolism and intracellular pH in a single hemisphere of the rat brain over an 8-h period following graded, traumatic, fluid percussion-induced brain injury. Following trauma the ratio of phosphocreatine to inorganic phosphate (PCr/Pi) declined in each injury group. This decline was transitory with low injury (1.0 +/- 0.5 atm), biphasic with moderate (2.1 +/- 0.4 atm) and high (3.9 +/- 0.9 atm) injury, and sustained following severe injury (5.9 +/- 0.7 atm). The initial PCr/Pi decline in the moderate and high injury groups was associated with intracellular acidosis; however, the second decline occurred in the absence of any pH changes. Alterations in ATP occurred only in severely injured animals and such changes were associated with marked acidosis and 100% mortality rate. After 4h, the posttraumatic PCr/Pi ratio correlated linearly with the severity of injury. We suggest that a reduced posttraumatic PCr/Pi ratio maymore » be indicative of altered mitochondrial energy production and may predict a reduced capacity of the cell to recover from traumatic injury.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of California, San Francisco (USA)
OSTI Identifier:
7025572
Resource Type:
Journal Article
Journal Name:
Magnet. Reson. Med.; (United States)
Additional Journal Information:
Journal Volume: 6:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; INJURIES; PHOSPHATES; METABOLISM; QUANTITY RATIO; PHOSPHOCREATINE; BLOOD PRESSURE; NUCLEAR MAGNETIC RESONANCE; PH VALUE; PHOSPHORUS 31; RATS; AMINO ACIDS; ANIMALS; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; RESONANCE; RODENTS; STABLE ISOTOPES; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Vink, R, McIntosh, T K, Yamakami, I, and Faden, A I. /sup 31/P NMR characterization of graded traumatic brain injury in rats. United States: N. p., 1988. Web. doi:10.1002/mrm.1910060105.
Vink, R, McIntosh, T K, Yamakami, I, & Faden, A I. /sup 31/P NMR characterization of graded traumatic brain injury in rats. United States. https://doi.org/10.1002/mrm.1910060105
Vink, R, McIntosh, T K, Yamakami, I, and Faden, A I. 1988. "/sup 31/P NMR characterization of graded traumatic brain injury in rats". United States. https://doi.org/10.1002/mrm.1910060105.
@article{osti_7025572,
title = {/sup 31/P NMR characterization of graded traumatic brain injury in rats},
author = {Vink, R and McIntosh, T K and Yamakami, I and Faden, A I},
abstractNote = {Irreversible tissue injury following central nervous system trauma is believed to result from both mechanical disruption at the time of primary insult, and more delayed autodestructive processes. These delayed events are associated with various biochemical changes, including alterations in phosphate energy metabolism and intracellular pH. Using /sup 31/P NMR, we have monitored the changes in phosphorus energy metabolism and intracellular pH in a single hemisphere of the rat brain over an 8-h period following graded, traumatic, fluid percussion-induced brain injury. Following trauma the ratio of phosphocreatine to inorganic phosphate (PCr/Pi) declined in each injury group. This decline was transitory with low injury (1.0 +/- 0.5 atm), biphasic with moderate (2.1 +/- 0.4 atm) and high (3.9 +/- 0.9 atm) injury, and sustained following severe injury (5.9 +/- 0.7 atm). The initial PCr/Pi decline in the moderate and high injury groups was associated with intracellular acidosis; however, the second decline occurred in the absence of any pH changes. Alterations in ATP occurred only in severely injured animals and such changes were associated with marked acidosis and 100% mortality rate. After 4h, the posttraumatic PCr/Pi ratio correlated linearly with the severity of injury. We suggest that a reduced posttraumatic PCr/Pi ratio may be indicative of altered mitochondrial energy production and may predict a reduced capacity of the cell to recover from traumatic injury.},
doi = {10.1002/mrm.1910060105},
url = {https://www.osti.gov/biblio/7025572}, journal = {Magnet. Reson. Med.; (United States)},
number = ,
volume = 6:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}