Genetic variation in erythrocyte membrane ion transport and possible relationship to essential hypertension

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7025571

Lee, J Y

Serial experiments were performed in order to understand and explore the Na/sup +/ transport system. In order to test possible covariation of cation and anion permeabilities, we applied inhibitors of cation or anion transport. Sulfonamide loop diuretics, furosemide and bumetanide, suppress 22/sub Na/sup +// influx into high permeability (HP) red cells but less into low permeability (LP) erythrocytes. These drugs also inhibit SO/sub 4/ = transport about 70% in both types of RBC. RBC pretreated with impermeant polyanions also show significantly decreased Na/sup +/ influx into HP but not LP RBC. However,a potent inhibitor of RBC anion transport, diisothiocyanostilbene disulfonate (DIDS), has no influence on Na/sup +/ transport. Since the glucose channel is another transmembrane protein in erythrocyte membranes, the effects of the potent glucose transport inhibitors, phlorizin and phloretinyl-3'-benzylazide (PBAz), were measured. Both chemicals effect reduction of Na/sup +/ flux. Because radioactive PBAz is not available, we employed another potent Na/sup +/ channel blocker, /sup 32/P-8-azido-ATP, in an attempt to label HP and LP RBC membranes. Autoradiograms showed that /sup 32/P labels only band 4.2 and external iodination with /sup 125/I yields similar results.

• Research Organization: Minnesota Univ., Minneapolis (USA)
• OSTI ID: 7025571
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PERMEABILITY
HYPERTENSION
PATHOGENESIS
SODIUM COMPOUNDS
MEMBRANE TRANSPORT
ATP
ERYTHROCYTES
GENETIC VARIABILITY
IODINE 125
PHOSPHORUS 32
SODIUM 22
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SODIUM ISOTOPES
SYMPTOMS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES
550901* - Pathology- Tracer Techniques