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Title: Atrial natriuretic peptide decreases blood volume in intact and anephric rats

Abstract

Atrial natriuretic peptide (ANP) reportedly lowers atrial pressure and increases hematocrit, suggesting venodilation and/or decreased blood volume (BV). To examine these possibilities, rat ANP (99-126) was administered to Inactinanesthetized rats (313 +/- 9 g, +/- SE) at 0.5 ..mu..g/kg/min for 30 minutes. Urine flow increased by 0.05 ml/min (p < 0.001) during the last 15 minutes of infusion. Mean arterial pressure (MAP) and thoracic central venous pressure (CVP) decreased (p < 0.001) by 12 and 0.5 mmHg, respectively; hematocrit increased by 4.1 units (p < 0.001) and BV (/sup 51/Cr-RBC) decreased by 3.4 ml/kg (p < 0.001). Mean circulatory filling pressure, measured by inflating an intracardiac balloon to briefly stop the circulation, did not change. Distribution of BV between the thoracic and spanchnic organs (whole-animal freezing in liquid nitrogen) was not measurably altered. The results suggest that the decrease in CVP was related more to decreased BV than to venodilation. To investigate possible mechanisms for the decreased BV, the same dose of ANP was administered to anephric rats. MAP decreased by 8 mmHg (p < 0.001); hematocrit increased by 2.4 units (p < 0.001) and BV decreased by 1.7 ml/kg (p < 0.05). The results indicate that short-term administration ofmore » ANP decreases blood volume by causing intravascular fluid to shift into the interstitium as well as by inducing diuresis.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Alton Ochsner Medical Foundation, New Orleans, LA
OSTI Identifier:
7024925
Alternate Identifier(s):
OSTI ID: 7024925
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ARTERIES; VASODILATION; PEPTIDES; BIOLOGICAL EFFECTS; VEINS; BLOOD COUNT; BLOOD PRESSURE; CARDIOVASCULAR DISEASES; CHROMIUM 51; ERYTHROCYTES; RATS; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BLOOD VESSELS; BODY; BODY FLUIDS; CARDIOVASCULAR SYSTEM; CHROMIUM ISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques; 550901 -- Pathology-- Tracer Techniques

Citation Formats

Trippodo, N.C., Chien, Y.W., Pegram, B.L., Cole, F.E., MacPhee, A.A., and Kardon, M.B. Atrial natriuretic peptide decreases blood volume in intact and anephric rats. United States: N. p., 1986. Web.
Trippodo, N.C., Chien, Y.W., Pegram, B.L., Cole, F.E., MacPhee, A.A., & Kardon, M.B. Atrial natriuretic peptide decreases blood volume in intact and anephric rats. United States.
Trippodo, N.C., Chien, Y.W., Pegram, B.L., Cole, F.E., MacPhee, A.A., and Kardon, M.B. Wed . "Atrial natriuretic peptide decreases blood volume in intact and anephric rats". United States.
@article{osti_7024925,
title = {Atrial natriuretic peptide decreases blood volume in intact and anephric rats},
author = {Trippodo, N.C. and Chien, Y.W. and Pegram, B.L. and Cole, F.E. and MacPhee, A.A. and Kardon, M.B.},
abstractNote = {Atrial natriuretic peptide (ANP) reportedly lowers atrial pressure and increases hematocrit, suggesting venodilation and/or decreased blood volume (BV). To examine these possibilities, rat ANP (99-126) was administered to Inactinanesthetized rats (313 +/- 9 g, +/- SE) at 0.5 ..mu..g/kg/min for 30 minutes. Urine flow increased by 0.05 ml/min (p < 0.001) during the last 15 minutes of infusion. Mean arterial pressure (MAP) and thoracic central venous pressure (CVP) decreased (p < 0.001) by 12 and 0.5 mmHg, respectively; hematocrit increased by 4.1 units (p < 0.001) and BV (/sup 51/Cr-RBC) decreased by 3.4 ml/kg (p < 0.001). Mean circulatory filling pressure, measured by inflating an intracardiac balloon to briefly stop the circulation, did not change. Distribution of BV between the thoracic and spanchnic organs (whole-animal freezing in liquid nitrogen) was not measurably altered. The results suggest that the decrease in CVP was related more to decreased BV than to venodilation. To investigate possible mechanisms for the decreased BV, the same dose of ANP was administered to anephric rats. MAP decreased by 8 mmHg (p < 0.001); hematocrit increased by 2.4 units (p < 0.001) and BV decreased by 1.7 ml/kg (p < 0.05). The results indicate that short-term administration of ANP decreases blood volume by causing intravascular fluid to shift into the interstitium as well as by inducing diuresis.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}

Conference:
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