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Title: Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase

Abstract

The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was affected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta-and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.

Authors:
;  [1]
  1. Univ. of Geneva (Switzerland)
Publication Date:
OSTI Identifier:
7017675
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (USA)
Additional Journal Information:
Journal Volume: 56:2; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; AROMATICS; BIODEGRADATION; GLYCOLS; BIOSYNTHESIS; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; OXYGENASES; PLASMIDS; PSEUDOMONAS; RECOMBINANT DNA; ALCOHOLS; BACTERIA; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; DNA; ENZYMES; HYDROXY COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; SYNTHESIS; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-)

Citation Formats

Wubbolts, M G, and Timmis, K N. Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase. United States: N. p., 1990. Web.
Wubbolts, M G, & Timmis, K N. Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase. United States.
Wubbolts, M G, and Timmis, K N. 1990. "Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase". United States.
@article{osti_7017675,
title = {Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase},
author = {Wubbolts, M G and Timmis, K N},
abstractNote = {The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was affected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta-and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.},
doi = {},
url = {https://www.osti.gov/biblio/7017675}, journal = {Applied and Environmental Microbiology; (USA)},
issn = {0099-2240},
number = ,
volume = 56:2,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 1990},
month = {Thu Feb 01 00:00:00 EST 1990}
}