Use of Congo red - polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7011547

Teather, R M; Wood, P J

The interaction of the direct dye Congo red with intact beta-D-glucans provides the basis for a rapid and sensitive assay system for bacterial strains possessing beta-(1 maps onto 4), (1 maps onto 3)-D-glucanohydrolase, beta-(1 maps onto 4)-D-glucanohydrolase, and beta-(1 maps onto 3)-D-glucanohydrolase activities. A close correspondence was observed between cellulolytic activity and beta-(1 maps onto 4)-D-glucanohydrolase and beta-(1 maps onto 4), (1 maps onto 3)-D-glucanohydrolase activities in isolates from the bovine rumen. Many of these isolates also possessed beta-(1 maps onto 3)-D-glucanohydrolase activity, and this characteristic may have taxonomic significance. (Refs. 19).

• Research Organization: Animal Research Centre and Food Research Inst Agriculture Canada, Ottawa, Ontario, Canada, K1A OC6
• OSTI ID: 7011547
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 43:4
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELLULOLYTIC ACTIVITY
MEASURING METHODS
CELLULOSE
BIODEGRADATION
DYES
POLYSACCHARIDES
RUMINANTS
STOMACH
ANIMALS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYME ACTIVITY
GASTROINTESTINAL TRACT
MAMMALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANS
SACCHARIDES
VERTEBRATES
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