Structure and mapping of the gene encoding mouse high affinity Fc[gamma]RI and chromosomal location of the human Fc[gamma]RI gene
- Austin Research Institute, Heidelberg (Australia)
- National Institute of Allergy and Infectious Diseases, Bethesda, MD (United States)
The authors describe the isolation and characterization of the gene encoding the mouse high affinity Fc receptor Fc[gamma]RI. Using a mouse cDNA Fc[gamma]RI probe four unique overlapping genomic clones were isolated and were found to encode the entire 9 kb of the mouse Fc[gamma]RI gene. Sequence analysis of the gene showed that six exons account for the entire Fc[gamma]RI cDNA sequences including the 5[prime] - and 3[prime]-untranslated sequences. The first and second exons encode the signal peptide; exons 3, 4, and 5 encode the extracellular Ig binding domains; and exon 6 encodes the transmembrane domain, the cytoplasmic region, and the entire 3[prime]-untranslated sequence. This exon pattern is similar to Fc[gamma]RII gene which contains 10 exons and encodes the b1 and b2 Fc[gamma]RII. Southern blot analysis has shown that the mouse Fc[gamma]RI gene is a single copy gene with no RFLP in inbred strains of mice, but analysis of an intersubspecies backcross of mice showed that unlike other mouse FcR genes which are on mouse chromosome 1 the locus encoding Fc[gamma]RI, termed Fcg1, is located on chromosome 3. Interestingly, the Fcg1 locus is located near the end of a region with known linkage homology to human chromosome 1. Analysis of human x rodent somatic cell hybrid cell lines indicates that the human FCG1 locus encoding the human Fc[gamma]RI maps to chromosome 1 and is therefore possibly linked to other FcR genes on this chromosome. These results suggest that the linkage relationships among these genes in the human genome are not preserved in the mouse. 30 refs., 4 figs., 2 tabs.
- OSTI ID:
- 7009449
- Journal Information:
- Journal of Immunology; (United States), Vol. 148:4; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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