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Title: The HLA class I gene family includes at least six genes and twelve pseudogenes and gene fragments

Abstract

The authors report the characterization of eight HLA class I homologous sequences isolated from cosmid and lambda libraries made from lymphoblastoid cell line 721 DNA. Four of these sequences, each contained within HindIII fragments of 1.7, 2.1, 3.0, and 8.0 kb, have class I homology extending over short intron-exon regions. The remaining four are found within 7.5-, 8.0-, 9.0-, and 16.0-kb HindIII fragments, the first having homology to the 5[prime] half of a class I gene whereas the latter three are homologous to the 3[prime] portion of a class I gene. When combined with the characterization of other class I clones, this work brings the total number of HLA class I homologous sequences cloned and characterized to 18. Restriction mapping of cosmid clones showed that some of these sequences are linked to one another and to other class I pseudogenes and genes within 50-kb regions. Reconstruction experiments using the 18 class I genes and pseudogenes were performed that indicated that all of the members of the HLA class I gene family detectable using HLA-A2 genomic DNA as probe had been cloned. An additional 19th member of the class I gene family was identified using an HLA-E cDNA probe. Further Southernmore » analysis with other class I probes indicated the 19 sequences comprise the entire class I gene family in LCL 721. Locus-specific probes were isolated from five of the eight clones and were used in Southern analysis of diverse genomic DNA to examine the polymorphism of the pseudogene sequences, demonstrating that some of them were highly polymorphic and some were missing entirely in certain haplotypes. An additional class I sequence, not contained within the 721 genome, was identified and may be found in association with the HLA-A11-Bw60 haplotype. Sequence comparisons were carried out to examine the evolutionary relationships among the pseudogenes. Hypothetical events in the evolution of the class I region are discussed. 59 refs., 8 figs., 4 tabs.« less

Authors:
 [1]; ;  [2];  [3]
  1. Fred Hutchinson Cancer Research Center, Seattle, WA (United States)
  2. Univ. of Minnesota, Minneapolis, MN (United States)
  3. Univ. of Washington, Seattle, WA (United States)
Publication Date:
OSTI Identifier:
7009405
Resource Type:
Journal Article
Journal Name:
Journal of Immunology; (United States)
Additional Journal Information:
Journal Volume: 149:6; Journal ID: ISSN 0022-1767
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; DETECTION; DNA SEQUENCING; HISTOCOMPATIBILITY COMPLEX; BIOLOGICAL EVOLUTION; DNA-CLONING; RFLPS; ANTIGENS; CLONING; DNA HYBRIDIZATION; HYBRIDIZATION; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Geraghty, D E, Koller, B H, Orr, H T, and Hansen, J A. The HLA class I gene family includes at least six genes and twelve pseudogenes and gene fragments. United States: N. p., 1992. Web.
Geraghty, D E, Koller, B H, Orr, H T, & Hansen, J A. The HLA class I gene family includes at least six genes and twelve pseudogenes and gene fragments. United States.
Geraghty, D E, Koller, B H, Orr, H T, and Hansen, J A. 1992. "The HLA class I gene family includes at least six genes and twelve pseudogenes and gene fragments". United States.
@article{osti_7009405,
title = {The HLA class I gene family includes at least six genes and twelve pseudogenes and gene fragments},
author = {Geraghty, D E and Koller, B H and Orr, H T and Hansen, J A},
abstractNote = {The authors report the characterization of eight HLA class I homologous sequences isolated from cosmid and lambda libraries made from lymphoblastoid cell line 721 DNA. Four of these sequences, each contained within HindIII fragments of 1.7, 2.1, 3.0, and 8.0 kb, have class I homology extending over short intron-exon regions. The remaining four are found within 7.5-, 8.0-, 9.0-, and 16.0-kb HindIII fragments, the first having homology to the 5[prime] half of a class I gene whereas the latter three are homologous to the 3[prime] portion of a class I gene. When combined with the characterization of other class I clones, this work brings the total number of HLA class I homologous sequences cloned and characterized to 18. Restriction mapping of cosmid clones showed that some of these sequences are linked to one another and to other class I pseudogenes and genes within 50-kb regions. Reconstruction experiments using the 18 class I genes and pseudogenes were performed that indicated that all of the members of the HLA class I gene family detectable using HLA-A2 genomic DNA as probe had been cloned. An additional 19th member of the class I gene family was identified using an HLA-E cDNA probe. Further Southern analysis with other class I probes indicated the 19 sequences comprise the entire class I gene family in LCL 721. Locus-specific probes were isolated from five of the eight clones and were used in Southern analysis of diverse genomic DNA to examine the polymorphism of the pseudogene sequences, demonstrating that some of them were highly polymorphic and some were missing entirely in certain haplotypes. An additional class I sequence, not contained within the 721 genome, was identified and may be found in association with the HLA-A11-Bw60 haplotype. Sequence comparisons were carried out to examine the evolutionary relationships among the pseudogenes. Hypothetical events in the evolution of the class I region are discussed. 59 refs., 8 figs., 4 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/7009405}, journal = {Journal of Immunology; (United States)},
issn = {0022-1767},
number = ,
volume = 149:6,
place = {United States},
year = {Tue Sep 15 00:00:00 EDT 1992},
month = {Tue Sep 15 00:00:00 EDT 1992}
}