Properties of a novel gene isolated from a Hodgkin's disease cell line that is expressed early during lymphoid cell activation
- Loyola Univ. Medical Center, Maywood, IL (United States) Hines Veterans Administration Hospital, IL (United States)
The authors have isolated a novel 667-bp cDNA clone, designated epag, from a Hodgkin's-disease cell line-derived library that is expressed in association with T cell activation and which is not related to any known gene family. By using reverse transcription/PCR, the authors have demonstrated that epag mRNA is expressed as early as 1 h after stimulation of normal PBMCs with anti-CD3. The levels of mRNA peaked by 4 h, and no expression was detectable by 12 h postactivation or in resting cells incubated in culture without activation. Expression of epag was also detected in PMA- and PHA-stimulated, but not in nonstimulated Jurkat cells, and overall its expression in transformed cell lines of hemopoietic origin is highly restricted. Sequence analysis of multiple independent cDNA clones showed that epag expressed in the Hodgkin's-disease cell line L428 is identical to the gene expressed in normal activated PBMC. Epag expression was detected by reverse transcription/PCR in RNA preparations made from various normal nonlymphoid tissues. Computer analysis of the sequence identified an open reading frame encoding a putative protein of 13.2 kDa initiating at a CUG translational codon. In vitro translation and Western blot analysis with anti-peptide serum supported this analysis. The authors hypothesize that epag functions as an early signal that helps mediate the activation of T cells. 63 refs., 11 figs.
- OSTI ID:
- 7009348
- Journal Information:
- Journal of Immunology; (United States), Vol. 152:5; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
GENE REGULATION
METABOLIC ACTIVATION
DNA SEQUENCING
DNA-CLONING
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
HYBRIDIZATION
LEUKOCYTES
MATERIALS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
551000 - Physiological Systems