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Title: Modulation of receptors and adenylate cyclase activity during sucrose feeding, food deprivation, and cold exposure

Abstract

Thermogenesis in brown adipose tissue (BAT) serves as a regulator of body temperature and weight maintenance. Thermogenesis can be stimulated by catecholamine activation of adenylate cyclase through the {beta}-adrenergic receptor. To investigate the effects of sucrose feeding, food deprivation, and cold exposure on the {beta}-adrenergic pathway, adenylate cyclase activity and {beta}-adrenergic receptors were assessed in rat BAT after 2 wk of sucrose feeding, 2 days of food deprivation, or 2 days of cold exposure. {beta}-Adrenergic receptors were identified in BAT using ({sup 125}I)iodocyanopindolol. Binding sites had the characteristics of mixed {beta}{sub 1}- and {beta}{sub 2}-type adrenergic receptors at a ratio of 60/40. After sucrose feeding or cold exposure, there was the expected increase in BAT mitochondrial mass as measured by total cytochrome-c oxidase activity but a decrease in {beta}-adrenergic receptor density due to a loss of the {beta}{sub 1}-adrenergic subtype. This BAT {beta}-adrenergic receptor downregulation was tissue specific, since myocardial {beta}-adrenergic receptors were unchanged with either sucrose feeding or cold exposure. Forskolin-stimulated adenylate cyclase activity increased in BAT after sucrose feeding or cold exposure but not after food deprivation. These data suggest that in BAT, sucrose feeding or cold exposure result in downregulation of {beta}-adrenergic receptors and that isoproterenol-stimulated adenylatemore » cyclase activity was limited by receptor availability.« less

Authors:
; ;  [1]
  1. Veterans Administration Medical Center, Los Angeles, CA (USA) Univ. of California, Los Angeles (USA)
Publication Date:
OSTI Identifier:
6994913
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 253:6; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYCLASES; ENZYME ACTIVITY; INDOLES; RADIORECEPTOR ASSAY; RECEPTORS; BIOCHEMICAL REACTION KINETICS; ADIPOSE TISSUE; BIOLOGICAL PATHWAYS; CATECHOLAMINES; DIET; FASTING; IODINE 125; PHYSIOLOGY; RATS; SACCHAROSE; TEMPERATURE DEPENDENCE; WEIGHT; AMINES; ANIMAL TISSUES; ANIMALS; AROMATICS; AZAARENES; AZOLES; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CONNECTIVE TISSUE; DAYS LIVING RADIOISOTOPES; DISACCHARIDES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LYASES; MAMMALS; MEMBRANE PROTEINS; NUCLEI; ODD-EVEN NUCLEI; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHENOLS; POLYPHENOLS; PROTEINS; PYRROLES; RADIOISOTOPES; REACTION KINETICS; RODENTS; SACCHARIDES; TISSUES; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Scarpace, P J, Baresi, L A, and Morley, J E. Modulation of receptors and adenylate cyclase activity during sucrose feeding, food deprivation, and cold exposure. United States: N. p., 1987. Web.
Scarpace, P J, Baresi, L A, & Morley, J E. Modulation of receptors and adenylate cyclase activity during sucrose feeding, food deprivation, and cold exposure. United States.
Scarpace, P J, Baresi, L A, and Morley, J E. 1987. "Modulation of receptors and adenylate cyclase activity during sucrose feeding, food deprivation, and cold exposure". United States.
@article{osti_6994913,
title = {Modulation of receptors and adenylate cyclase activity during sucrose feeding, food deprivation, and cold exposure},
author = {Scarpace, P J and Baresi, L A and Morley, J E},
abstractNote = {Thermogenesis in brown adipose tissue (BAT) serves as a regulator of body temperature and weight maintenance. Thermogenesis can be stimulated by catecholamine activation of adenylate cyclase through the {beta}-adrenergic receptor. To investigate the effects of sucrose feeding, food deprivation, and cold exposure on the {beta}-adrenergic pathway, adenylate cyclase activity and {beta}-adrenergic receptors were assessed in rat BAT after 2 wk of sucrose feeding, 2 days of food deprivation, or 2 days of cold exposure. {beta}-Adrenergic receptors were identified in BAT using ({sup 125}I)iodocyanopindolol. Binding sites had the characteristics of mixed {beta}{sub 1}- and {beta}{sub 2}-type adrenergic receptors at a ratio of 60/40. After sucrose feeding or cold exposure, there was the expected increase in BAT mitochondrial mass as measured by total cytochrome-c oxidase activity but a decrease in {beta}-adrenergic receptor density due to a loss of the {beta}{sub 1}-adrenergic subtype. This BAT {beta}-adrenergic receptor downregulation was tissue specific, since myocardial {beta}-adrenergic receptors were unchanged with either sucrose feeding or cold exposure. Forskolin-stimulated adenylate cyclase activity increased in BAT after sucrose feeding or cold exposure but not after food deprivation. These data suggest that in BAT, sucrose feeding or cold exposure result in downregulation of {beta}-adrenergic receptors and that isoproterenol-stimulated adenylate cyclase activity was limited by receptor availability.},
doi = {},
url = {https://www.osti.gov/biblio/6994913}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 253:6,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 1987},
month = {Tue Dec 01 00:00:00 EST 1987}
}