Thromboxane A/sub 2//prostaglandin H/sub 2/ receptor interactions in human platelets

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6994821

Kattelman, E J

This work investigated the interactions of several compounds with the TXA/sub 2//PGH/sub 2/ receptor in intact human platelets using direct ligand binding techniques. The compound studied were: (1) U46619, a chemically stable TXA/sub 2//PGH/sub 2/ mimetic; (2) four putative TXA/sub 2//PGH/sub 2/ receptor antagonists (namely SQ 29, 548, ONO 3708, BM 13.177 and 13-azaprostanoic acid); and (3) I-APA-PhN/sub 3/, a derivative of 13-azaprostanoic acid which incorporates a photoactive iodophenylazide group. Using (/sup 3/H)-U46619 as the radioligand, a binding assay was developed for use in intact, unactivated human platelets.

• Research Organization: Illinois Univ., Chicago (USA)
• OSTI ID: 6994821
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROSTAGLANDINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
BLOOD PLATELETS
LIGANDS
MAN
RADIOASSAY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques