Bacteroides gingivalis and Bacteroides intermedius recognize different sites on human fibrinogen

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Journal of Bacteriology; (USA)

OSTI ID:6993290

Lantz, M S; Allen, R D; Bounelis, P; Switalski, L M; Hook, M ^[1]

---

1. Univ. of Alabama, Birmingham (USA)

Bacteroides (Porphyromonas) gingivalis and Bacteroides (Porphyromonas) intermedius have been implicated in the etiology of human periodontal diseases. These organisms are able to bind and degrade human fibrinogen, and these interactions may play a role in the pathogenesis of periodontal disease. In attempts to map the bacterial binding sites along the fibrinogen molecule, we have found that strains of B. gingivalis and B. intermedius, respectively, recognize spatially distant and distinct sites on the fibrinogen molecule. Isolated reduced and alkylated alpha-, beta-, and gamma-fibrinogen chains inhibited binding of 125I-fibrinogen to both Bacteroides species in a concentration-dependent manner. Plasmin fragments D and to some extent fragment E, however, produced a concentration-dependent inhibition of 125I-fibrinogen binding to B. intermedius strains but did not affect binding of 125I-fibrinogen to B. gingivalis strains. Radiolabeled fibrinogen chains and fragments were compared with 125I-fibrinogen with respect to specificity and reversibility of binding to bacteria. According to these criteria, gamma chain most closely resembled the native fibrinogen molecule in behavior toward B. gingivalis strains and fragments D most closely resembled fibrinogen in behavior toward B. intermedius strains. The ability of anti-human fibrinogen immunoglobulin G (IgG) to inhibit binding of 125I-fibrinogen to B. intermedius strains was greatly reduced by absorbing the IgG with fragments D. Absorbing the IgG with fragments D had no effect on the ability of the antibody to inhibit binding of 125I-fibrinogen to B. gingivalis strains. A purified staphylococcal fibrinogen-binding protein blocked binding of 125I-fibrinogen to B. intermedius strains but not to B. gingivalis strains.

• OSTI ID: 6993290
• Journal Information: Journal of Bacteriology; (USA), Vol. 172:2; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIAL DISEASES
PATHOGENESIS
FIBRINOGEN
RECEPTORS
ORAL CAVITY
BIOCHEMICAL REACTION KINETICS
IMMUNOGLOBULINS
INHIBITION
IODINE 125
ISOTOPE DILUTION
MAN
SENSE ORGANS DISEASES
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques