Effects of X-ray irradiation on natural killer (NK) cell system. II. Increased sensitivity to natural killer cytotoxic factor (NKCF)

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Immunopharmacology and Immunotoxicology; (USA)

DOI:https://doi.org/10.3109/08923978909005382· OSTI ID:6992753

Uchida, A; Mizutani, Y; Nagamuta, M; Ikenaga, M ^[1]

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1. Kyoto Univ. (Japan)

Irradiation with low-doses of X-rays of tumor cells elevated their susceptibility to lysis by natural killer (NK) cells in an accompanying paper. Cytotoxicity assays conducted at the single cell level revealed that X-ray irradiation of K562 cells did not affect the number of effector-target conjugates but increased the frequency of dead conjugated target cells. During interaction with K562 cells large granular lymphocytes released a soluble cytotoxic factor (NKCF) that killed the target cells. X-ray irradiation did not affect the NKCF stimulatory ability of K562 cells, while it elevated their sensitivity to the lytic effect of NKCF. In contrast to X-rays, exposure to ultraviolet (UV) radiation of K562 cells did not elevate their NK sensitivity but rather reduced it. Treatment with mitomycin C produced no effect on NK sensitivity. These results indicate that X-ray irradiation elevates the target sensitivity to NKCF, which may be involved in the increased NK sensitivity, and that the X-ray effect may be different from that of UV radiation or DNA synthesis inhibition.

• OSTI ID: 6992753
• Journal Information: Immunopharmacology and Immunotoxicology; (USA), Vol. 11:2-3; ISSN 0892-3973
• Country of Publication: United States
• Language: English

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