Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]
The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified ..cap alpha..-1,4-endopolygalacturonase (EPG). The EPG-treated SEPS were fractionated by anion-exchange and gel-permeation chromatography. The following polysaccharides were found: xyloglucan, arabinoxylan, at least two arabinogalactans, a rhamnogalacturonan-II-like polysaccharide, and a polygalacturonic acid-rich polysaccharide. The oligogalacturonide fragments expected from EPG-digested homogalacturonan were also identified. Evidence was obtained for the presence of a rhamnogalacturonan-I-like polysaccharide. All of the above polysaccharides have been isolated from or are believed to be present in sycamore cell walls. Furthermore, all of the noncellulosic polysaccharides known to be present in sycamore cell-walls appears to be present in the SEPS.
- Research Organization:
- Univ. of Colorado, Boulder
- DOE Contract Number:
- AC02-76ER01426
- OSTI ID:
- 6991584
- Journal Information:
- Plant Physiol.; (United States), Vol. 80:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL WALL
STRUCTURAL CHEMICAL ANALYSIS
PLANT CELLS
POLYSACCHARIDES
BIOCHEMISTRY
CELL CULTURES
ENZYMATIC HYDROLYSIS
FRACTIONATION
SYCAMORES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
HYDROLYSIS
LYSIS
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
TREES
550300* - Cytology