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Title: Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]

Abstract

The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified ..cap alpha..-1,4-endopolygalacturonase (EPG). The EPG-treated SEPS were fractionated by anion-exchange and gel-permeation chromatography. The following polysaccharides were found: xyloglucan, arabinoxylan, at least two arabinogalactans, a rhamnogalacturonan-II-like polysaccharide, and a polygalacturonic acid-rich polysaccharide. The oligogalacturonide fragments expected from EPG-digested homogalacturonan were also identified. Evidence was obtained for the presence of a rhamnogalacturonan-I-like polysaccharide. All of the above polysaccharides have been isolated from or are believed to be present in sycamore cell walls. Furthermore, all of the noncellulosic polysaccharides known to be present in sycamore cell-walls appears to be present in the SEPS.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Colorado, Boulder
OSTI Identifier:
6991584
DOE Contract Number:  
AC02-76ER01426
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 80:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL WALL; STRUCTURAL CHEMICAL ANALYSIS; PLANT CELLS; POLYSACCHARIDES; BIOCHEMISTRY; CELL CULTURES; ENZYMATIC HYDROLYSIS; FRACTIONATION; SYCAMORES; CARBOHYDRATES; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; HYDROLYSIS; LYSIS; ORGANIC COMPOUNDS; PLANTS; SACCHARIDES; SEPARATION PROCESSES; SOLVOLYSIS; TREES; 550300* - Cytology

Citation Formats

Stevenson, T T, McNeil, M, Darvill, A G, and Albersheim, P. Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]. United States: N. p., 1986. Web.
Stevenson, T T, McNeil, M, Darvill, A G, & Albersheim, P. Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]. United States.
Stevenson, T T, McNeil, M, Darvill, A G, and Albersheim, P. Tue . "Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]". United States.
@article{osti_6991584,
title = {Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]},
author = {Stevenson, T T and McNeil, M and Darvill, A G and Albersheim, P},
abstractNote = {The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified ..cap alpha..-1,4-endopolygalacturonase (EPG). The EPG-treated SEPS were fractionated by anion-exchange and gel-permeation chromatography. The following polysaccharides were found: xyloglucan, arabinoxylan, at least two arabinogalactans, a rhamnogalacturonan-II-like polysaccharide, and a polygalacturonic acid-rich polysaccharide. The oligogalacturonide fragments expected from EPG-digested homogalacturonan were also identified. Evidence was obtained for the presence of a rhamnogalacturonan-I-like polysaccharide. All of the above polysaccharides have been isolated from or are believed to be present in sycamore cell walls. Furthermore, all of the noncellulosic polysaccharides known to be present in sycamore cell-walls appears to be present in the SEPS.},
doi = {},
journal = {Plant Physiol.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = {1986},
month = {4}
}