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Title: Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae

Abstract

In the experiments described here, batchwise fermentations were employed with Candida shehatae cells induced by growth on either glucose or xylose, and fermentation kinetic constants were determined. Results show that ethanol production rates were higher with xylose-grown inocula than with glucose-grown inocula. This comparison held true for all of the combinations of glucose and xylose tested. The ethanol production rate was highest for a fermentation of 6% xylose supplemented with 3% glucose by xylose-grown inoculum. The next highest rates were obtained with the fermentation of pure glucose and the glucose-xylose mixture by xylose-grown inoculum. Ethanol yields did not appear to be greatly affected by the sugars tested. The rates of mannose, glucose, xylose, galactose, and L-arabinose utilization by xylose-grown inocula were determined in a separate experiment. Mannose (6%) was used at a higher rate than any of the other sugars tested. Mannose also showed the highest rate of ethanol production. Most of the mannose taken up, however, could not be accounted for in the ethanol produced, so it is possible that significant amounts of mannitol were formed. The D-galactose was fermented at a significantly lower rate than glucose, xylose, or mannose. The L-arabinose was not consumed. The ethanol fermentation ratemore » observed was much lower with an autoclaved acid hydrolysate than with the conditioned hydrolysate or a mixture of individual sugars. Contacting the hydrolysate with cells for an extended period of time prior to autoclaving greatly improved fermentability, but this strain of C. shehatae was still very susceptible to inhibition by components in the hydrolysate (Table III).« less

Authors:
;
Publication Date:
Research Org.:
Department of Agriculture, Madison, WI (USA)
OSTI Identifier:
6986643
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 31:5
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; CANDIDA; PRODUCTIVITY; ETHANOL; BIOSYNTHESIS; GLUCOSE; FERMENTATION; XYLOSE; BATCH CULTURE; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; FUNGI; HEXOSES; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; PLANTS; SACCHARIDES; SYNTHESIS; YEASTS; 090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)

Citation Formats

Jeffries, T W, and Sreenath, H K. Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae. United States: N. p., 1988. Web. doi:10.1002/bit.260310516.
Jeffries, T W, & Sreenath, H K. Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae. United States. doi:10.1002/bit.260310516.
Jeffries, T W, and Sreenath, H K. Tue . "Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae". United States. doi:10.1002/bit.260310516.
@article{osti_6986643,
title = {Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae},
author = {Jeffries, T W and Sreenath, H K},
abstractNote = {In the experiments described here, batchwise fermentations were employed with Candida shehatae cells induced by growth on either glucose or xylose, and fermentation kinetic constants were determined. Results show that ethanol production rates were higher with xylose-grown inocula than with glucose-grown inocula. This comparison held true for all of the combinations of glucose and xylose tested. The ethanol production rate was highest for a fermentation of 6% xylose supplemented with 3% glucose by xylose-grown inoculum. The next highest rates were obtained with the fermentation of pure glucose and the glucose-xylose mixture by xylose-grown inoculum. Ethanol yields did not appear to be greatly affected by the sugars tested. The rates of mannose, glucose, xylose, galactose, and L-arabinose utilization by xylose-grown inocula were determined in a separate experiment. Mannose (6%) was used at a higher rate than any of the other sugars tested. Mannose also showed the highest rate of ethanol production. Most of the mannose taken up, however, could not be accounted for in the ethanol produced, so it is possible that significant amounts of mannitol were formed. The D-galactose was fermented at a significantly lower rate than glucose, xylose, or mannose. The L-arabinose was not consumed. The ethanol fermentation rate observed was much lower with an autoclaved acid hydrolysate than with the conditioned hydrolysate or a mixture of individual sugars. Contacting the hydrolysate with cells for an extended period of time prior to autoclaving greatly improved fermentability, but this strain of C. shehatae was still very susceptible to inhibition by components in the hydrolysate (Table III).},
doi = {10.1002/bit.260310516},
journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 31:5,
place = {United States},
year = {1988},
month = {4}
}