Fermentation of hemicellulosic sugars and sugar mixtures by Candida shehatae
In the experiments described here, batchwise fermentations were employed with Candida shehatae cells induced by growth on either glucose or xylose, and fermentation kinetic constants were determined. Results show that ethanol production rates were higher with xylose-grown inocula than with glucose-grown inocula. This comparison held true for all of the combinations of glucose and xylose tested. The ethanol production rate was highest for a fermentation of 6% xylose supplemented with 3% glucose by xylose-grown inoculum. The next highest rates were obtained with the fermentation of pure glucose and the glucose-xylose mixture by xylose-grown inoculum. Ethanol yields did not appear to be greatly affected by the sugars tested. The rates of mannose, glucose, xylose, galactose, and L-arabinose utilization by xylose-grown inocula were determined in a separate experiment. Mannose (6%) was used at a higher rate than any of the other sugars tested. Mannose also showed the highest rate of ethanol production. Most of the mannose taken up, however, could not be accounted for in the ethanol produced, so it is possible that significant amounts of mannitol were formed. The D-galactose was fermented at a significantly lower rate than glucose, xylose, or mannose. The L-arabinose was not consumed. The ethanol fermentation rate observed was much lower with an autoclaved acid hydrolysate than with the conditioned hydrolysate or a mixture of individual sugars. Contacting the hydrolysate with cells for an extended period of time prior to autoclaving greatly improved fermentability, but this strain of C. shehatae was still very susceptible to inhibition by components in the hydrolysate (Table III).
- Research Organization:
- Department of Agriculture, Madison, WI (USA)
- OSTI ID:
- 6986643
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 31:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CANDIDA
PRODUCTIVITY
ETHANOL
BIOSYNTHESIS
GLUCOSE
FERMENTATION
XYLOSE
BATCH CULTURE
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
FUNGI
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
SACCHARIDES
SYNTHESIS
YEASTS
090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)