Removal of. cap alpha. -tocopherol from blood and its comparison with other lipids: studies of inhibition
To investigate the mechanism of ..cap alpha..-tocopherol (AT) uptake into tissues, loss of /sup 3/H-AT from blood was characterized and compared with the losses of two major blood lipids: /sup 3/H-cholesterol (CHO) and /sup 3/H-trioleoylglycerol (TO). Male Long-Evans rats (200-325 gm) were injected with serum labelled with one lipid, and bled from the tail from 1-240 min. In one group heparin (HEP), an inhibitor of lipoprotein lipase which mediates uptake of TO into tissues, was intravenously injected prior to serum and following corn oil gavage. The control group was only gavaged (GAV). A third group was injected with labelled serum which had been incubated with 1,2-cyclohexanedione (CHD), a reagent which modifies the receptors responsible for removal of CHO-rich low density lipoproteins from blood. Labelled serum incubated only with borate buffer (BO) was injected into the fourth group. HEP slowed TO loss from 2-220 min, but left CHO loss unchanged. AT loss was slowed by HEP from 100 min on. That AT responded to HEP but over a time span different from that of TO suggests that AT may be removed by a mechanism distinct from that of TO but sensitive to HEP. CHD slowed CHO loss from 40-240 min while TO and AT loss were uninhibited. This argues against a mechanism of removal common to both AT and CHO.
- Research Organization:
- Univ. of Rochester, NY
- DOE Contract Number:
- FG02-85ER60282
- OSTI ID:
- 6985915
- Report Number(s):
- CONF-8606151-; TRN: 86-039095
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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VITAMIN E
MEMBRANE TRANSPORT
REMOVAL
BLOOD
CHOLESTEROL
COMPARATIVE EVALUATIONS
GLYCEROL
HEPARIN
INHIBITION
LIPIDS
RATS
REAGENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
ANIMALS
ANTICOAGULANTS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
DRUGS
HEMATOLOGIC AGENTS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
STEROIDS
STEROLS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques