Anatomical and pharmacological characterization of excitatory amino acid receptors
The majority of the excitatory neurotransmission in the vertebrate Central Nervous System is thought to be mediated by acidic amino acid neurotransmitters. However, relatively little is known about the excitatory amino acid receptors and their distribution within the CNS. By analyzing radioligand binding to purified synaptic plasma membranes and to thin tissue sections processed for autoradiography, multiple distinct binding sites were found. These binding sites exhibited the pharmacological properties indicative of the excitatory amino acid receptors, which had been identified by electrophysiological techniques. Specifically, L-(/sup 3/H)-glutamate and D-(/sup 3/H)-amino-5-phosphonopentanoate appear to label N-methyl-D-aspartate receptors, L-(/sup 3/H)-glutamate and (/sup 3/H)-kainic acid appear to label kainic acid receptors, and L-(/sup 3/H)-glutamate and (/sup 3/H)-amino-3-hydroxy-5-methyl-4-isoxazolepropionate appear to label quisqualate receptors. Together, these results confirm the three receptor scheme proposed for excitatory amino acid neurotransmission. These results also show that these transmitter-receptor systems are differentially distributed in the brain, and that the total distribution is consistent with that found by other markers for excitatory amino acid-using neurons.
- Research Organization:
- California Univ., Irvine (USA)
- OSTI ID:
- 6985369
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
PHARMACOLOGY
RECEPTORS
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
ASPARTIC ACID
BIOELECTRICITY
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
GLUTAMIC ACID
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DISTRIBUTION
ELECTRICITY
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
551001* - Physiological Systems- Tracer Techniques