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Title: [Regulation of terpene metabolism]

Abstract

During the last grant period, we have completed studies on the key pathways of monoterpene biosynthesis and catabolism in sage and peppermint, and have, by several lines of evidence, deciphered the rate-limiting step of each pathway. We have at least partially purified and characterized the relevant enzymes of each pathway. We have made a strong case, based on analytical, in vivo, and in vitro studies, that terpene accumulation depends upon the balance between biosynthesis and catabolism, and provided supporting evidence that these processes are developmentally-regulated and very closely associated with senescence of the oil glands. Oil gland ontogeny has been characterized at the ultrastructural level. We have exploited foliar-applied bioregulators to delay gland senescence, and have developed tissue explant and cell culture systems to study several elusive aspects of catabolism. We have isolated pure gland cell clusters and localized monoterpene biosynthesis and catabolism within these structures, and have used these preparations as starting materials for the purification to homogeneity of target regulatory'' enzymes. We have thus developed the necessary background knowledge, based on a firm understanding of enzymology, as well as the necessary experimental tools for studying the regulation of monoterpene metabolism at the molecular level. Furthermore, we are nowmore » in a position to extend our systematic approach to other terpenoid classes (C[sub 15]-C[sub 30]) produced by oil glands.« less

Authors:
Publication Date:
Research Org.:
Washington State Univ., Pullman, WA (United States)
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6984681
Report Number(s):
DOE/ER/13869-3
ON: DE93010768
DOE Contract Number:  
FG06-88ER13869
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ESSENTIAL OILS; BIOSYNTHESIS; TERPENES; BIOLOGICAL PATHWAYS; CAMPHOR; CARBON 14 COMPOUNDS; CYCLASES; OXIDOREDUCTASES; PROGRESS REPORT; TISSUE CULTURES; TRACER TECHNIQUES; CARBON COMPOUNDS; DOCUMENT TYPES; ENZYMES; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; LYASES; OILS; ORGANIC COMPOUNDS; OTHER ORGANIC COMPOUNDS; PROTEINS; SYNTHESIS; 090900* - Biomass Fuels- Processing- (1990-); 550201 - Biochemistry- Tracer Techniques; 550501 - Metabolism- Tracer Techniques

Citation Formats

Croteau, R. [Regulation of terpene metabolism]. United States: N. p., 1991. Web. doi:10.2172/6984681.
Croteau, R. [Regulation of terpene metabolism]. United States. doi:10.2172/6984681.
Croteau, R. Tue . "[Regulation of terpene metabolism]". United States. doi:10.2172/6984681. https://www.osti.gov/servlets/purl/6984681.
@article{osti_6984681,
title = {[Regulation of terpene metabolism]},
author = {Croteau, R},
abstractNote = {During the last grant period, we have completed studies on the key pathways of monoterpene biosynthesis and catabolism in sage and peppermint, and have, by several lines of evidence, deciphered the rate-limiting step of each pathway. We have at least partially purified and characterized the relevant enzymes of each pathway. We have made a strong case, based on analytical, in vivo, and in vitro studies, that terpene accumulation depends upon the balance between biosynthesis and catabolism, and provided supporting evidence that these processes are developmentally-regulated and very closely associated with senescence of the oil glands. Oil gland ontogeny has been characterized at the ultrastructural level. We have exploited foliar-applied bioregulators to delay gland senescence, and have developed tissue explant and cell culture systems to study several elusive aspects of catabolism. We have isolated pure gland cell clusters and localized monoterpene biosynthesis and catabolism within these structures, and have used these preparations as starting materials for the purification to homogeneity of target regulatory'' enzymes. We have thus developed the necessary background knowledge, based on a firm understanding of enzymology, as well as the necessary experimental tools for studying the regulation of monoterpene metabolism at the molecular level. Furthermore, we are now in a position to extend our systematic approach to other terpenoid classes (C[sub 15]-C[sub 30]) produced by oil glands.},
doi = {10.2172/6984681},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}