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Title: Xylose fermentation to ethanol

Abstract

The past several years have seen tremendous progress in the understanding of xylose metabolism and in the identification, characterization, and development of strains with improved xylose fermentation characteristics. A survey of the numerous microorganisms capable of directly fermenting xylose to ethanol indicates that wild-type yeast and recombinant bacteria offer the best overall performance in terms of high yield, final ethanol concentration, and volumetric productivity. The best performing bacteria, yeast, and fungi can achieve yields greater than 0.4 g/g and final ethanol concentrations approaching 5%. Productivities remain low for most yeast and particularly for fungi, but volumetric productivities exceeding 1.0 g/L-h have been reported for xylose-fermenting bacteria. In terms of wild-type microorganisms, strains of the yeast Pichia stipitis show the most promise in the short term for direct high-yield fermentation of xylose without byproduct formation. Of the recombinant xylose-fermenting microorganisms developed, recombinant E. coli ATTC 11303 (pLOI297) exhibits the most favorable performance characteristics reported to date.

Authors:
Publication Date:
Research Org.:
National Renewable Energy Lab., Golden, CO (United States)
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6975389
Report Number(s):
NREL/TP-421-4944
ON: DE93000067
DOE Contract Number:  
AC02-83CH10093
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; XYLOSE; FERMENTATION; ANAEROBIC CONDITIONS; BACTERIA; BIOLOGICAL PATHWAYS; CULTURE MEDIA; ENZYME INDUCTION; ETHANOL; GENETIC ENGINEERING; MEMBRANE TRANSPORT; OPTIMIZATION; RECOMBINANT DNA; REVIEWS; YEASTS; ALCOHOLS; ALDEHYDES; BIOCONVERSION; BIOTECHNOLOGY; CARBOHYDRATES; DNA; DOCUMENT TYPES; EUMYCOTA; FUNGI; GENE REGULATION; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PENTOSES; PLANTS; SACCHARIDES; 090900* - Biomass Fuels- Processing- (1990-)

Citation Formats

McMillan, J D. Xylose fermentation to ethanol. United States: N. p., 1993. Web. doi:10.2172/6975389.
McMillan, J D. Xylose fermentation to ethanol. United States. doi:10.2172/6975389.
McMillan, J D. Fri . "Xylose fermentation to ethanol". United States. doi:10.2172/6975389. https://www.osti.gov/servlets/purl/6975389.
@article{osti_6975389,
title = {Xylose fermentation to ethanol},
author = {McMillan, J D},
abstractNote = {The past several years have seen tremendous progress in the understanding of xylose metabolism and in the identification, characterization, and development of strains with improved xylose fermentation characteristics. A survey of the numerous microorganisms capable of directly fermenting xylose to ethanol indicates that wild-type yeast and recombinant bacteria offer the best overall performance in terms of high yield, final ethanol concentration, and volumetric productivity. The best performing bacteria, yeast, and fungi can achieve yields greater than 0.4 g/g and final ethanol concentrations approaching 5%. Productivities remain low for most yeast and particularly for fungi, but volumetric productivities exceeding 1.0 g/L-h have been reported for xylose-fermenting bacteria. In terms of wild-type microorganisms, strains of the yeast Pichia stipitis show the most promise in the short term for direct high-yield fermentation of xylose without byproduct formation. Of the recombinant xylose-fermenting microorganisms developed, recombinant E. coli ATTC 11303 (pLOI297) exhibits the most favorable performance characteristics reported to date.},
doi = {10.2172/6975389},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}