Detection of genetic changes in Barrett's adenocarcinoma and Barrett's esophagus by DNA in situ hybridization and immunohistochemistry

Journal Article · Tue Feb 01 00:00:00 EST 1994 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990150212· OSTI ID:6974455

Krishnadath, K K; Tilanus, H W; Alers, J C; Mulder, A H; Dekken, H van ^[1]

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1. Erasmus Univ., Rotterdam (Netherlands)

The authors have investigated the occurrence of chromosomal DNA and cell cycle-related protein changes in Barrett's epithelium and adenocarcinoma. The presence of numerical chromosomal aberrations was studied by applying nonisotopic in situ hybridization (ISH) with (peri-)centromeric DNA probes, specific for chromosomes 7, 8, 17, and Y, to routinely processed tissue sections of five cases (4 male, 1 female) of Barrett's adenocarcinoma and adjacent Barrett's epithelium. Cell cycle-related protein expression was studied by immunohistochemistry (IHC) for p53 protein and the Ki-67 antigen (Mib-1) in subsequent sections. P53 protein overexpression was found in 3 of the 5 tumors. Overrepresentation of chromosome 8 and loss of chromosome 17 were found in 2 adenocarcinomas, both also negative for p53 protein overexpression. Y-loss, mostly clonal, was detected in 3 of the 4 male adenocarcinomas and 2 cases of adjacent Barrett's epithelium. One tumor had both areas of overrepresentation and loss of the Y chromosome. All Barrett's adenocarcinomas appeared to contain aneuploid cell populations. No relation was found between cell proliferation characteristics and chromosomal aberrations. They conclude that ISH with chromosome specific DNA probes can be applied for the assessment of potentially important numerical chromosome changes in Barrett's esophagus. Further, the combination of IHC and ISH is useful for evaluation of specific genetic events. 41 refs., 3 figs., 2 tabs.

• OSTI ID: 6974455
• Journal Information: Cytometry (Baltimore); (United States), Vol. 15:2; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARCINOMAS
DIAGNOSIS
PATHOGENESIS
EPITHELIUM
CHROMOSOMAL ABERRATIONS
ESOPHAGUS
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 7
HUMAN CHROMOSOME 8
HUMAN Y CHROMOSOME
ANIMAL TISSUES
BODY
CHROMOSOMES
DIGESTIVE SYSTEM
DISEASES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
MUTATIONS
NEOPLASMS
ORGANS
TISSUES
Y CHROMOSOME
550400* - Genetics
550900 - Pathology