Optimization of a flow cell interface for reverse-phase liquid chromatography/Fourier transform infrared spectrometry
The further development of a method for on-line aqueous-based reversed-phase high-performance liquid chromatography/Fourier transform infrared spectrometry is described. An improved interface device based upon the postcolumn extraction of solutes into a proper analyte fluid has been constructed. In this fashion, the coupling of the separation to the spectrometer maintains the developed chromatographic resolution and yet retains the spectral integrity of each analyte with modest interferences. The device allows the detection of solutes within the time frame of the chromatographic separation. The apparent limit of detection for compounds containing carbonyl functionalities is on the order of micrograms per component as minimum injectable quantities.
- Research Organization:
- Virginia Polytechnic Institute and State Univ., Blacksburg
- DOE Contract Number:
- FG22-84PC70799
- OSTI ID:
- 6971208
- Journal Information:
- Anal. Chem.; (United States), Vol. 59:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CARBONYLS
INFRARED SPECTRA
LIQUID COLUMN CHROMATOGRAPHY
FOURIER TRANSFORM SPECTROMETERS
PERFORMANCE
EXPERIMENTAL DATA
OPTIMIZATION
QUANTITATIVE CHEMICAL ANALYSIS
TRACE AMOUNTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
INFORMATION
MEASURING INSTRUMENTS
NUMERICAL DATA
SEPARATION PROCESSES
SPECTRA
SPECTROMETERS
400102* - Chemical & Spectral Procedures