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Title: [Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]

Abstract

A major goal of this project is to use a combined molecular genetic, biochemical and physiological approach to understand the relationship between photosynthetic performance and the structure of the multifunctional D1 reaction center protein of Photosystem II encoded by the chloroplast psbA gene. Relative to other chloroplast proteins, turover of D1 is rapid and highly light dependent and de novo synthesis of D1 is required for a plant's recovery from short term exposure to irradiances which induce photoinhibitory damage. These observations have led to models for a damage/repair cycle of PSII involving the targeted degradation and replacement of photodamaged D1. To investigate the effects of perturbing the D1 cycle on photosynthesis and autotrophic growth under high and low irradiance, we have examined the consequences of site-specific mutations of the psbA and 16S rRNA genes affecting synthesis, maturation and function/stability of the D1 protein introduced into the chloroplast genome of wildtype strain of the green alga Chlamydomonas reinhardtii using biolistic transformation.

Publication Date:
Research Org.:
Duke Univ., Durham, NC (United States)
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6969011
Report Number(s):
DOE/ER/14005-T2
ON: DE93004365
DOE Contract Number:  
FG05-89ER14005
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 14 SOLAR ENERGY; THYLAKOID MEMBRANE PROTEINS; GENE MUTATIONS; PHOSPHORYLATION; BIOLOGICAL HALF-LIFE; CARBON 13; DNA SEQUENCING; MUTANTS; OXYGEN 18; PHOTOSYNTHETIC REACTION CENTERS; PROGRESS REPORT; RIBOSOMAL RNA; RNA PROCESSING; SULFUR 35; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DOCUMENT TYPES; EVEN-EVEN NUCLEI; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MEMBRANE PROTEINS; MUTATIONS; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN ISOTOPES; PROTEINS; RADIOISOTOPES; RNA; STABLE ISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques; 140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

. [Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]. United States: N. p., 1992. Web. doi:10.2172/6969011.
. [Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]. United States. https://doi.org/10.2172/6969011
. 1992. "[Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]". United States. https://doi.org/10.2172/6969011. https://www.osti.gov/servlets/purl/6969011.
@article{osti_6969011,
title = {[Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]},
author = {},
abstractNote = {A major goal of this project is to use a combined molecular genetic, biochemical and physiological approach to understand the relationship between photosynthetic performance and the structure of the multifunctional D1 reaction center protein of Photosystem II encoded by the chloroplast psbA gene. Relative to other chloroplast proteins, turover of D1 is rapid and highly light dependent and de novo synthesis of D1 is required for a plant's recovery from short term exposure to irradiances which induce photoinhibitory damage. These observations have led to models for a damage/repair cycle of PSII involving the targeted degradation and replacement of photodamaged D1. To investigate the effects of perturbing the D1 cycle on photosynthesis and autotrophic growth under high and low irradiance, we have examined the consequences of site-specific mutations of the psbA and 16S rRNA genes affecting synthesis, maturation and function/stability of the D1 protein introduced into the chloroplast genome of wildtype strain of the green alga Chlamydomonas reinhardtii using biolistic transformation.},
doi = {10.2172/6969011},
url = {https://www.osti.gov/biblio/6969011}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}