Cometabolism of chlorinated solvents and binary chlorinated solvent mixtures using M. trichosporium OB3b PP358
- Univ. of Texas, Austin (United States)
The mutant methanotroph, Methylosinus trichosporium OB3b PP358, which constitutively expresses soluble methane monooxygenase (sMMO), was used to study the degradation kinetics of individual chlorinated solvents and binary solvent mixtures. Although sMMO`s broad specificity permits a wide range of chlorinated solvents to be degraded, it creates the potential for competitive inhibition of degradation rates in mixtures because multiple chemicals are simultaneously available to the enzyme. To effectively design both exsitu and in-situ groundwater bioremediation systems using strain PP358, kinetic parameters for chlorinated solvent degradation and accurate kinetic expressions to account for inhibition in mixtures are required. Toward this end, the degradation parameters for six prevalent chlorinated solvents an the verification of enzyme competition model for binary mixtures were the focus of this investigation. M.trichosporium OB3b PP258 degraded trichloroethylene (TCE), chloroform, cis-1,2-dichloroethylene (c-DCE), trans-1,2-dichloroethylene (t-DCE), and 1,1-dichloroethylene (1,1-DCE) rapidly, with maximum substrate transformation rates of {gt}20.8,3.1,9.5 24.8, and {gt}7.5 mg/mg-day, respectively. 1,1,1-trichloroethane (TCA) was not significantly degraded.
- OSTI ID:
- 696817
- Journal Information:
- Biotechnology and Bioengineering, Vol. 65, Issue 1; Other Information: PBD: 5 Oct 1999
- Country of Publication:
- United States
- Language:
- English
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