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Title: Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies

Abstract

The aim of this work was to answer the following questions: (i) can the authors realize a long-term dechlorination with a pure anaerobic strain in soil and (ii) can the authors monitor the process on an adequate scale with a controlled simulator of in situ conditions. The soil bioreactor was fed continuously with 3-chlorobenzonate (3-CB) as a model chloroaromatic compound. Bioaugmentation was carried out by inoculating Desulfomonile tiedjei in localized areas in the bioreactor. Temporal and spatial distribution of the 3-CB dechlorination activity was investigated with specific biological activity tests for approximately 4 months following the inoculation. These tests involved the minimally invasive sampling of geometrically distinct points in the reactor and their off-site handling within reconstructed microcosms, allowing the assessment of dechlorinating and methanogenic soil activities. Using autoclaved and nonautoclaved agricultural soil, the results showed a heterogeneous distribution of the dechlorination activity in the bioreactor. The autoclaved soil expressed a high microbial activity as reflected by biogas production and 3-CB dechlorination. Furthermore, durable establishment of D. tiedjei in both autoclaved and nonautoclaved soil was shown, although in the latter portion of the reactor the microorganism was maintained only at the top surface.

Authors:
 [1]; ; ;  [2];  [3];  [4]
  1. Univ. Catholique de Louvain, Louvain-la-Neuve (Belgium). Unit of Bioengineering|[Univ. of Ghent (Belgium)
  2. Univ. Catholique de Louvain, Louvain-la-Neuve (Belgium). Unit of Bioengineering
  3. Univ. of Ghent (Belgium). Lab. of Microbial Ecology
  4. Univ. Catholique de Louvain (Belgium). Lab. of Microbial Genetics
Publication Date:
OSTI Identifier:
696773
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 33; Journal Issue: 17; Other Information: PBD: 1 Sep 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; SOILS; BIODEGRADATION; BIOREACTORS; ANAEROBIC CONDITIONS; REMEDIAL ACTION; CHLORINATED AROMATIC HYDROCARBONS; PERFORMANCE TESTING; SULFATE-REDUCING BACTERIA

Citation Formats

Fantroussi, S.E., Belkacemi, M., Naveau, H., Agathos, S.N., Top, E.M., and Mahillon, J. Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies. United States: N. p., 1999. Web. doi:10.1021/es981353p.
Fantroussi, S.E., Belkacemi, M., Naveau, H., Agathos, S.N., Top, E.M., & Mahillon, J. Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies. United States. doi:10.1021/es981353p.
Fantroussi, S.E., Belkacemi, M., Naveau, H., Agathos, S.N., Top, E.M., and Mahillon, J. Wed . "Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies". United States. doi:10.1021/es981353p.
@article{osti_696773,
title = {Bioaugmentation of a soil bioreactor designed for pilot-scale anaerobic bioremediation studies},
author = {Fantroussi, S.E. and Belkacemi, M. and Naveau, H. and Agathos, S.N. and Top, E.M. and Mahillon, J.},
abstractNote = {The aim of this work was to answer the following questions: (i) can the authors realize a long-term dechlorination with a pure anaerobic strain in soil and (ii) can the authors monitor the process on an adequate scale with a controlled simulator of in situ conditions. The soil bioreactor was fed continuously with 3-chlorobenzonate (3-CB) as a model chloroaromatic compound. Bioaugmentation was carried out by inoculating Desulfomonile tiedjei in localized areas in the bioreactor. Temporal and spatial distribution of the 3-CB dechlorination activity was investigated with specific biological activity tests for approximately 4 months following the inoculation. These tests involved the minimally invasive sampling of geometrically distinct points in the reactor and their off-site handling within reconstructed microcosms, allowing the assessment of dechlorinating and methanogenic soil activities. Using autoclaved and nonautoclaved agricultural soil, the results showed a heterogeneous distribution of the dechlorination activity in the bioreactor. The autoclaved soil expressed a high microbial activity as reflected by biogas production and 3-CB dechlorination. Furthermore, durable establishment of D. tiedjei in both autoclaved and nonautoclaved soil was shown, although in the latter portion of the reactor the microorganism was maintained only at the top surface.},
doi = {10.1021/es981353p},
journal = {Environmental Science and Technology},
number = 17,
volume = 33,
place = {United States},
year = {1999},
month = {9}
}