Determination of chromium in urine by stable isotope dilution gas chromatography/mass spectrometry using lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent
- Univ. of Virginia Health Sciences Center, Charlottesville (USA)
An isotope dilution gas chromatography/mass spectrometry method using lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent is described for the determination of chromium in urine. A wet digestion procedure with HNO3-H2O2 is used for oxidizing the organic matter associated with urine samples. The isotope ratios are measured by selected ion monitoring in a general-purpose mass spectrometer using a 10-m fused silica capillary column. Memory effect, in sequential analyses of samples with different isotope ratios, was evaluated by preparing a series of synthetic mixtures and was found to be negligible. The accuracy of the method was verified by quantitation of chromium in the NIST freeze-dried urine reference material, SRM-2670, with a recommended chromium concentration of 13 micrograms/L in the normal level and certified chromium concentration of 85 +/- 6 micrograms/L in the elevated level.
- OSTI ID:
- 6967318
- Journal Information:
- Analytical Chemistry (Washington); (USA), Vol. 62:2; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHROMIUM
METABOLISM
CHELATING AGENTS
GAS CHROMATOGRAPHY
ISOTOPE DILUTION
ISOTOPE RATIO
MAN
MASS SPECTROSCOPY
STABLE ISOTOPES
ANIMALS
CHROMATOGRAPHY
ELEMENTS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALS
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques