Stimulus specific effect of ibuprofen on chemiluminescence of sheep neutrophils
The authors have shown that pretreatment with ibuprofen inhibits free radical release from complement stimulated neutrophils. To further examine the effect of ibuprofen on neutrophil free radical release, they stimulated neutrophils with the synthetic peptide, FMLP, phorbol myristate acetate (PMA), or zymosan-activated plasma (ZAP). Pure (>95%), viable (>95%) sheep neutrophils (2 x 10/sup 6/) were placed in HEPES buffer, luminol, drug or vehicle and stimulated in the luminometer with one of the stimuli. The chemiluminescence (CL) response was recorded and the drug treated samples were compared to vehicle treated controls. Ibuprofen had a dose dependent effect on CL in ZAP stimulated neutrophils. At the highest dose (10/sup -2/M) these cells produced only 37 +/- 7% of the CL response observed in the control cells. In contrast, at the same dose, ibuprofen did not significantly attenuate CL seen in FMLP stimulated cells, with these cells producing 79 +/- 7% of the control cells; nor did ibuprofen effect PMA stimulated CL, as these cells produced a CL response that was 85 +/- 8% of the control cells. Ibuprofen appears to have a stimulus specific effect on free radical release in activated neutrophils. It is also apparent that ibuprofen inhibits complement stimulated free radical release by some mechanism independent of its cyclooxygenase inhibitory effect.
- Research Organization:
- Jefferson Medical College, Philadelphia, PA
- OSTI ID:
- 6962228
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition by nonsteroidal antiinflammatory drugs of luminol-dependent human-granulocyte chemiluminescence and /sup 3/H FMLP binding. Effect of sulindac sulfide, indomethacin metabolite, and optical enantiomers (+) and (-) MK830
Amiloride (Am) dissociates human neutrophil (N) activation events
Related Subjects
ANTIPYRETICS
BIOLOGICAL EFFECTS
NEUTROPHILS
CHEMILUMINESCENCE
PHORBOL ESTERS
RADICALS
SECRETION
DOSE-RESPONSE RELATIONSHIPS
INHIBITION
PEPTIDES
SHEEP
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DOMESTIC ANIMALS
DRUGS
ESTERS
LEUKOCYTES
LUMINESCENCE
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
RUMINANTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology