Expression of a new antigen, Tml on T cells after modulation by anti-T3 antibodies of IgM class
During the modulation of the T3/Ti complex by anti-T3 antibody, 235, of IgM class, a new antigen, Tml was detected with an IgGl mAb produced against mAb 235 treated T cells. This antigen was inducible only on T cells modulated by IgM anti-T3 (mAb 235 and mAb 38.1) and not by anti-T3 of other isotypes. Tml was a 33Kd molecule which was detectable in a small percentage of T cells and B cells but not on granulocytes, platelets, erythrocytes and thymocytes. Significantly, monocytes displayed this antigen on their cell surfaces. While not detectable on leukemic T cells HPB-ALL and Jurkat, Tml was expressed when these cell lines were modulated with 235. T cells activated by PHA, Con A, TPA, PWM, PPD, and allo-antigens showed little increase in the expression of Tml. In contrast, B cells activated by S. aureus or goat anti-mu antibodies along with BCGF showed increased expression of this antigen. Attempts to precipitate Tml from activated B cells have not been successful. On T cells, Tml appeared after 30 minutes of T3 modulation, before the disappearance of T3 antigen. Cycloheximide, a protein synthesis inhibitor, did not block Tml expression, suggesting that the molecule was present before the modulation process. The expression of Tml is induced uniquely by IgM anti-T3 mAbs. Perturbation of the membrane by these mAbs may have allowed the emergence of an intracellular antigen, Tml, on the cell surface.
- Research Organization:
- Oklahoma Medical Research Foundation, Oklahoma City
- OSTI ID:
- 6962224
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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