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Title: Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells

Abstract

This paper studies adenylate cyclase acticvity in Ehrlich's ascites carcinoma (EAC) cells during administration of drugs with a hypolipidemic action. Seven to eight days before they were killed, male mice ingested the antidiabetic biguanide phenformin, and the phospholipid-containing preparation Essentiale in drinking water. The cAMP formed was isolated by chromatography on Silufol plates after incubation of the enzyme preparation with tritium-ATP, or was determined by the competitive binding method with protein. It is shown that despite the possible differences in the concrete mechanism of action of the hypolipidemic agents chosen for study on the cyclase system, the use of such agents, offers definite prospects for oriented modification of the hormone sensitivity of tumor cells.

Authors:
; ;
Publication Date:
Research Org.:
Ministry of Health of The USSR, Leningrad
OSTI Identifier:
6958313
Alternate Identifier(s):
OSTI ID: 6958313
Resource Type:
Journal Article
Journal Name:
Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
Additional Journal Information:
Journal Volume: 100:9
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASCITES TUMOR CELLS; SENSITIVITY; CYCLASES; ENZYME ACTIVITY; DRUGS; BIOLOGICAL EFFECTS; RADIORECEPTOR ASSAY; AMP; ATP; EHRLICH ASCITES TUMOR; HORMONES; PHOSPHOLIPIDS; TRANSPLANTS; TRITIUM COMPOUNDS; ANIMAL CELLS; ENZYMES; ESTERS; EXPERIMENTAL NEOPLASMS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; LYASES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; TRACER TECHNIQUES; TUMOR CELLS 550301* -- Cytology-- Tracer Techniques; 550201 -- Biochemistry-- Tracer Techniques

Citation Formats

Bershtein, L.M., Kovaleva, I.G., and Rozenberg, O.A. Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells. United States: N. p., 1986. Web.
Bershtein, L.M., Kovaleva, I.G., & Rozenberg, O.A. Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells. United States.
Bershtein, L.M., Kovaleva, I.G., and Rozenberg, O.A. Sat . "Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells". United States.
@article{osti_6958313,
title = {Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells},
author = {Bershtein, L.M. and Kovaleva, I.G. and Rozenberg, O.A.},
abstractNote = {This paper studies adenylate cyclase acticvity in Ehrlich's ascites carcinoma (EAC) cells during administration of drugs with a hypolipidemic action. Seven to eight days before they were killed, male mice ingested the antidiabetic biguanide phenformin, and the phospholipid-containing preparation Essentiale in drinking water. The cAMP formed was isolated by chromatography on Silufol plates after incubation of the enzyme preparation with tritium-ATP, or was determined by the competitive binding method with protein. It is shown that despite the possible differences in the concrete mechanism of action of the hypolipidemic agents chosen for study on the cyclase system, the use of such agents, offers definite prospects for oriented modification of the hormone sensitivity of tumor cells.},
doi = {},
journal = {Bull. Exp. Biol. Med. (Engl. Transl.); (United States)},
number = ,
volume = 100:9,
place = {United States},
year = {1986},
month = {2}
}