Functional size of photosynthetic electron transport chain determined by radiation inactivation

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.1.158· OSTI ID:6957002

Pan, R S; Chen, L F; Wang, M Y; Tsal, M Y; L, Pan R; Hsu, B D

Radiation inactivation technique was employed to determine the functional size of photosynthetic electron transport chain of spinach chloroplasts. The functional size for photosystem I+II(H/sub 2/O to methylviologen) was 623 +/- 37 kilodaltons; for photosystem II (H/sub 2/O to dimethylquinone/ferricyanide), 174 +/- 11 kilodaltons; and for photosystem I (reduced diaminodurene to methylviologen), 190 +/- 11 kilodaltons. The difference between 364 +/- 22 (the sum of 174 +/- 11 and 190 +/- 11) kilodaltons and 623 +/- 37 kilodaltons is partially explained to be due to the presence of two molecules of cytochrome b/sub 6//f complex of 280 kilodaltons. The molecular mass for other partial reactions of photosynthetic electron flow, also measured by radiation inactivation, is reported. The molecular mass obtained by this technique is compared with that determined by other conventional biochemical methods. A working hypothesis for the composition, stoichiometry, and organization of polypeptides for photosynthetic electron transport chain is proposed.

• Research Organization: National Tsing Hua Univ., Hsin Chu (Taiwan)
• OSTI ID: 6957002
• Journal Information: Plant Physiol.; (United States), Vol. 85:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLOROPLASTS
BIOLOGICAL RADIATION EFFECTS
THYLAKOID MEMBRANE PROTEINS
PHOTOSYNTHETIC REACTION CENTERS
MOLECULAR WEIGHT
COBALT 60
CYTOCHROMES
ELECTRON TRANSFER
INACTIVATION
POLYPEPTIDES
SPINACH
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
COBALT ISOTOPES
FOOD
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MEMBRANE PROTEINS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PIGMENTS
PLANTS
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
VEGETABLES
YEARS LIVING RADIOISOTOPES
550200* - Biochemistry
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture