Accelerator mass spectrometry in the biomedical sciences: Applications in low-exposure biomedical and environmental dosimetry
We are utilizing Accelerator Mass Spectrometry (AMS) as a sensitive detector for tracking the disposition of radio-isotope labeled molecules in the biomedical sciences. These applications have shown the effectiveness of AMS as a tool to quantify biologically important molecules at extremely low levels. For example, AMS is being used to determine the amount of carcinogen covalently bound to animal DNA (DNA adduct) at levels relevent to human exposure. Studies have been undertaken in our laboratory utilizing heterocyclic amine food-borne carcinogens and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a potent environmental carcinogen, to study the metabolism of carcinogens at low doses. In addition, AMS is being used to detect the presence of rare proteins (mutant forms of protamine) in human sperm. Problems in the life sciences where detection sensitivity or sample sizes are limitations should also benefit from AMS. Studies are underway to measure the molecular targeting of cancer chemotherapeutics in human tissue and to pursue applications for receptor biology. We are also applying other candidate isotopes, such as {sup 3}H (double labeling with {sup 14}C) and {sup 41}Ca (bone absorption) to problems in biology. The results described here with {sup 14}C-labeled molecules coupled with new isotope applications clearly show AMS technology to be an important new tool for the biomedical sciences community. 29 refs., 5 figs., 2 tabs.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- CANSERC; DOE/DP; NCI; NIEHS
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6947352
- Report Number(s):
- UCRL-JC-103998; ON: DE90012989; CNN: 222Y-01-ES-70158 CA40811
- Country of Publication:
- United States
- Language:
- English
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37 INORGANIC
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PHYSICAL AND ANALYTICAL CHEMISTRY
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DIOXIN
DNA ADDUCTS
DOSIMETRY
PROTAMINES
ACCELERATOR FACILITIES
ALUMINIUM 26
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CARBON 14
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOASSAY
CALCIUM ISOTOPES
CARBON ISOTOPES
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COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEASURING INSTRUMENTS
NUCLEI
NUCLEOPROTEINS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PROTEINS
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RADIOIMMUNODETECTION
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