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Title: Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma

Abstract

This study provides the first direct experimental evidence that human malignant gliomas secrete soluble polypeptides with TGF-like activity. The conditioned medium from three well-characterized malignant glioma cell lines promote the growth of NRK indicator cells in soft agar. Following acid extraction and gel filtration, TGF-like activity was recovered from all three cell lines. Active fractions from gel filtration in two of the glioma cell lines (U-343 MG-A and SF-210) were further purified by reverse-phase HPLC. The TGF isolated from U-343 MG-A by HPLC is an acid- and heat-stable protein complex whose activity is destroyed by reducing agents and incubation with monospecific anti-TGF-alpha monoclonal antibodies. The NRK colony stimulating activity of this TGF is potentiated by the addition of TGF-beta. The partially purified U-343 MG-A TGF competes with radiolabeled (/sup 125/I)-ECF for the EGF-receptor on A431 epidermoid carcinoma cells. Finally, a total RNA preparation from U-343- MG-A contains a 4.8 kilobase mRNA for TGF-alpha. Therefore, U-343 MG-A secretes a soluble polypeptide with TGF-alpha-like activity. In contrast, the purified SF-210 malignant glioma cell line secretes an acid- and heat-stable TGF with neither TGF-alpha- nor TGF-beta-like activity.

Authors:
Publication Date:
Research Org.:
California Univ., San Francisco (USA)
OSTI Identifier:
6945395
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLIOMAS; PATHOGENESIS; GROWTH FACTORS; BIOLOGICAL FUNCTIONS; CHEMICAL COMPOSITION; TUMOR CELLS; COLONY FORMATION; IODINE 125; LIQUID COLUMN CHROMATOGRAPHY; MAN; POLYPEPTIDES; TRACER TECHNIQUES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; FUNCTIONS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MITOGENS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDES; PRIMATES; PROTEINS; RADIOISOTOPES; SEPARATION PROCESSES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Rutka, J T. Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma. United States: N. p., 1987. Web.
Rutka, J T. Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma. United States.
Rutka, J T. 1987. "Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma". United States.
@article{osti_6945395,
title = {Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma},
author = {Rutka, J T},
abstractNote = {This study provides the first direct experimental evidence that human malignant gliomas secrete soluble polypeptides with TGF-like activity. The conditioned medium from three well-characterized malignant glioma cell lines promote the growth of NRK indicator cells in soft agar. Following acid extraction and gel filtration, TGF-like activity was recovered from all three cell lines. Active fractions from gel filtration in two of the glioma cell lines (U-343 MG-A and SF-210) were further purified by reverse-phase HPLC. The TGF isolated from U-343 MG-A by HPLC is an acid- and heat-stable protein complex whose activity is destroyed by reducing agents and incubation with monospecific anti-TGF-alpha monoclonal antibodies. The NRK colony stimulating activity of this TGF is potentiated by the addition of TGF-beta. The partially purified U-343 MG-A TGF competes with radiolabeled (/sup 125/I)-ECF for the EGF-receptor on A431 epidermoid carcinoma cells. Finally, a total RNA preparation from U-343- MG-A contains a 4.8 kilobase mRNA for TGF-alpha. Therefore, U-343 MG-A secretes a soluble polypeptide with TGF-alpha-like activity. In contrast, the purified SF-210 malignant glioma cell line secretes an acid- and heat-stable TGF with neither TGF-alpha- nor TGF-beta-like activity.},
doi = {},
url = {https://www.osti.gov/biblio/6945395}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}

Thesis/Dissertation:
Other availability
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