Early changes in protein expression of barley following inoculation with erysiphe graminis f. sp. hordei

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6945229

Simons, S P; Somerville, S C ^[1]

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1. Michigan State Univ., East Lansing (USA)

Erysiphe graminis f. sp. hordei is an obligate pathogen of barley causing the powdery mildew disease. Resistance to this disease is the product of a highly specific interaction between barley lines with specific resistance alleles and pathogen races carrying complementary avirulence alleles. Using congenic barley lines which differ at the M1-a disease reaction locus, we hope to define the early molecular events of this interaction. Accordingly, resistant and susceptible barley seedlings were labelled with {sup 35}S-methionine and examined by two-dimensional electrophoresis at two hour intervals following inoculation. Infection related changes were observed with both isolines during the four to twelve hour time period. Additional differences existed constitutively between the barley lines. These differences have been quantified. Further characterization of these proteins will yield useful markers for events preceding or coinciding with cytological responses any may lead to identification and cloning of the M1-a gene.

• OSTI ID: 6945229
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PATHOGENS
INFECTIVITY
PROTEINS
GENES
BARLEY
LABELLED COMPOUNDS
METHIONINE
PHYSIOLOGY
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GRASS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques