Minimum number of lipids are required to support the functional properties of the nicotinic acetylcholine receptor
The detergent sodium cholate was used to both solubilize and partially delipidate the nicotinic acetylcholine receptor from Torpedo californica. Using both native membranes and reconstituted membranes, it is shown that the detergent to lipid molar ratio is the most important parameter in determining the effect of the detergent on the functional properties of the receptor. Receptor-lipid complexes were quantitatively separated from detergent and excess lipids by centrifugation through detergent-free sucrose gradients. The lipid to protein molar ratio of the complexes could be precisely controlled by adjusting the cholate and lipid concentrations of the starting membranes. Analyses of both ion influx activity and ligand binding revealed that a minimum of 45 lipids per receptor was required for stabilization of the receptor in a fully functional state. Progressive irreversible inactivation occurred as the lipid to protein mole ratio was decreased below 45, and complete inactivation occurred below a ratio of 20. The results are consistent with a functional requirement for a single shell of lipids around the perimeter of the receptor.
- Research Organization:
- Univ. of California, Davis (USA)
- OSTI ID:
- 6938943
- Journal Information:
- Biochemistry; (United States), Vol. 27:10
- Country of Publication:
- United States
- Language:
- English
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RECEPTORS
BIOCHEMISTRY
TOXINS
RADIORECEPTOR ASSAY
ACETYLCHOLINE
CENTRIFUGATION
IODINE 125
LIGANDS
LIPIDS
TRANSMISSION ELECTRON MICROSCOPY
AMINES
AMMONIUM COMPOUNDS
ANTIGENS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ESTERS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
MEMBRANE PROTEINS
MICROSCOPY
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SEPARATION PROCESSES
TOXIC MATERIALS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics