Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent

Journal Article · Fri Aug 29 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(86)80107-3· OSTI ID:6937294

Rossier, M F; Dentand, I A; Lew, P D; Capponi, A M; Vallotton, M B

The metabolism of (/sub 3/H)inositol (1,4,5)-trisphosphate was followed in permeabilized bovine adrenal glomerulosa cells. At low Ca++ concentration (pCa = 7.2), more than 90% of (/sub 3/H)inositol (1,4,5)-trisphosphate had disappeared within 2 min, while two other metabolites, (/sub 3/H)inositol (1,3,4)-trisphosphate and (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate appeared progressively. At higher Ca++ concentrations (pCa = 5.7 and 4.8), the formation of these two metabolites was markedly increased, but completely abolished if the medium was ATP-depleted. The peak levels for the generation of (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate (1 min) preceded those of (3H)inositol (1,3,4)-trisphosphate and were closely correlated. These results suggest that, in adrenal glomerulosa cells, the isomer inositol (1,3,4)-trisphosphate is generated from inositol (1,4,5)-trisphosphate via a calcium-sensitive and ATP-dependent phosphorylation/dephosphorylation pathway involving the formation of inositol (1,3,4,5)-tetrakisphosphate.

• Research Organization: Univ. Hospital, Geneva, Switzerland
• OSTI ID: 6937294
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INOSITOLS
METABOLISM
ADRENAL GLANDS
ANIMAL CELLS
CALCIUM
METABOLITES
PHOSPHATES
PHOSPHORYLATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
ELEMENTS
ENDOCRINE GLANDS
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques