Comparative effects of endothelin and phorbol 12-13 dibutyrate in rat aorta

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90580-8· OSTI ID:6936423

Auguet, M; Delaflotte, S; Chabrier, P E; Braquet, P ^[1]

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1. Institut Henri Beaufour Research Labs., Cedex (France)

The vasoconstrictive properties of endothelin (ET-1) and the protein kinase C activator, phorbol 12-13 dibutyrate (PDB) were comparatively investigated in isolated rat aorta. ET-1 and PDB induced a slowly developing sustained contraction in endothelium denuded aorta. Maximal contractions induced by ET-1 and PDB were unaffected by diltiazem. Substantial contraction to ET-1 and PDB remained in calcium-free medium. Contractions of ET-1 and PDB in calcium-free medium were unaffected by intracellular calcium depletion induced by phenylephrine. Following the response to ET-1 and PDB in a calcium-free medium, an additional sustained was observed after calcium was added to the bath. The protein kinase C inhibitor, H7 was more potent in inhibiting contractions induced by phenylephrine and KCl than the ones elicited by ET-1 and PDB. The other protein kinase C inhibitors i.e. staurosporine and phloretin inhibited to a similar extent all the agonists tested. These results suggest that protein kinase C may play an important role in mediating the contraction to ET-1 in rat aorta.

• OSTI ID: 6936423
• Journal Information: Life Sciences; (USA), Vol. 45:21; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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VASOCONSTRICTION
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PHOSPHOTRANSFERASES
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