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Title: Characterization of 47D10, a glycoprotein associated with pancreatic carcinoma

Abstract

A mouse monoclonal antibody (MAb), 47D10, was raised against a human lung adenocarcinoma cell line, A549. 47D10 bound to lines derived from breast, colon, lung, and pancreatic tumors, but not to normal fibroblasts. Granulocytes also expressed 47D10 but red blood cells and lymphocytes were negative. Immunoperoxidase staining of paraffin-embedded tissues indicated that 47D10 was reactive with 36 out of 38 cases of pancreatic adenocarcinoma, but not with chronic pancreatitis, regenerative pancreas or normal pancreas. The 47D10 antigen was a group of surface glycoproteins ranging from 63-97Kd (average Mr 85Kd). The antigen could be labeled by /sup 35/S-methionine, /sup 125/I, tritiated glucosamine, fucose, and mannose. Pulse-chase labeling showed that the 63-97Kd mature antigens were derived from precursors of 63Kd and 65Kd. The mature antigen was sensitive to endoglycosidase F (endo F) whereas the precursors were susceptible to both endoglycosidase H and endo F. Endo F digestion resulted in a polypeptide of 38Kd. Therefore, the 47D10 antigen is composed of at least 55% N-linked carbohydrates. The 47D10 MAb seems to be distinct from previously identified MAb against pancreatic tumors and may be useful in the diagnosis of pancreatic carcinoma.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
E.I. Du Pont de Nemours and Co., Billerica, MA
OSTI Identifier:
6936200
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIGENS; CHEMICAL COMPOSITION; CARCINOMAS; ANTIGEN-ANTIBODY REACTIONS; MONOCLONAL ANTIBODIES; PANCREAS; GLUCOPROTEINS; IODINE 125; LARGE INTESTINE; LUNGS; MAMMARY GLANDS; MAN; METHIONINE; MICE; SULFUR 35; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; EVEN-ODD NUCLEI; GASTROINTESTINAL TRACT; GLANDS; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RADIOISOTOPES; RESPIRATORY SYSTEM; RODENTS; SACCHARIDES; SULFUR ISOTOPES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Ho, M K, Kato, K P, Murray, J H, Wolfe, H, Rabin, H, and Carney, W P. Characterization of 47D10, a glycoprotein associated with pancreatic carcinoma. United States: N. p., 1986. Web.
Ho, M K, Kato, K P, Murray, J H, Wolfe, H, Rabin, H, & Carney, W P. Characterization of 47D10, a glycoprotein associated with pancreatic carcinoma. United States.
Ho, M K, Kato, K P, Murray, J H, Wolfe, H, Rabin, H, and Carney, W P. Wed . "Characterization of 47D10, a glycoprotein associated with pancreatic carcinoma". United States.
@article{osti_6936200,
title = {Characterization of 47D10, a glycoprotein associated with pancreatic carcinoma},
author = {Ho, M K and Kato, K P and Murray, J H and Wolfe, H and Rabin, H and Carney, W P},
abstractNote = {A mouse monoclonal antibody (MAb), 47D10, was raised against a human lung adenocarcinoma cell line, A549. 47D10 bound to lines derived from breast, colon, lung, and pancreatic tumors, but not to normal fibroblasts. Granulocytes also expressed 47D10 but red blood cells and lymphocytes were negative. Immunoperoxidase staining of paraffin-embedded tissues indicated that 47D10 was reactive with 36 out of 38 cases of pancreatic adenocarcinoma, but not with chronic pancreatitis, regenerative pancreas or normal pancreas. The 47D10 antigen was a group of surface glycoproteins ranging from 63-97Kd (average Mr 85Kd). The antigen could be labeled by /sup 35/S-methionine, /sup 125/I, tritiated glucosamine, fucose, and mannose. Pulse-chase labeling showed that the 63-97Kd mature antigens were derived from precursors of 63Kd and 65Kd. The mature antigen was sensitive to endoglycosidase F (endo F) whereas the precursors were susceptible to both endoglycosidase H and endo F. Endo F digestion resulted in a polypeptide of 38Kd. Therefore, the 47D10 antigen is composed of at least 55% N-linked carbohydrates. The 47D10 MAb seems to be distinct from previously identified MAb against pancreatic tumors and may be useful in the diagnosis of pancreatic carcinoma.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {1986},
month = {3}
}

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