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Title: Biological production of hydrogen

Abstract

All Phase I objectives were satisfactorily achieved. Stoichiometric yields of hydrogen were obtained from CO with R. rubrum. Fast growth was observed on synthesis gas only; however, growth was maximized with a small amount of yeast extract at 30[degrees]C and pH 7. Intrinsic kinetic parameters were determined and showed that fast hydrogen production rates are possible. The Phase II research program concentrated on defining the optimal culture and bioreactor for hydrogen production. To insure that the best possible system was developed, cultures were screened for enzyme activity and hydrogen production. Promising cultures were optimized for hydrogen yield and CO conversion. Bioreactors that achieved high mass transfer rates, as well as high cell concentrations, were studied. Advanced bioreactor concepts, such as solid-state fermentation, non-aqueous fermentation, and high pressure fermentation were applied to these reactors to enhance mass transport. Process design and economic evaluations of the various alternatives were used to guide the research program. A new bacterial culture has been isolated from natural sources which consumes CO rapidly and gives high hydrogen yields. The new isolate, ERIH2, is able to utilize a variety of sugars as growth substrates and, unlike R. rubrum, does not require light for growth. The yield ofmore » H[sub 2] by ERIH2 reach 100 percent. Typical H[sub 2] yields by R. rubrum were 75--85 percent.« less

Authors:
; ;
Publication Date:
Research Org.:
Engineering Resources, Inc., Fayetteville, AR (United States)
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6928226
Report Number(s):
DOE/ER/81057-93/C0107; CONF-920951-15
ON: DE93002604
DOE Contract Number:  
FG05-90ER81057
Resource Type:
Conference
Resource Relation:
Conference: US Department of Energy contractors review meeting on gasification and gas stream cleanup systems, Morgantown, WV (United States), 15-17 Sep 1992
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; 01 COAL, LIGNITE, AND PEAT; 59 BASIC BIOLOGICAL SCIENCES; HYDROGEN PRODUCTION; BIOSYNTHESIS; BIOREACTORS; BIOTECHNOLOGY; CATALYSTS; CONVERSION; HYDROGEN SULFIDES; SHIFT PROCESSES; SYNTHESIS GAS; WATER GAS PROCESSES; CHALCOGENIDES; CHEMICAL REACTIONS; FLUIDS; GASES; HYDROGEN COMPOUNDS; SULFIDES; SULFUR COMPOUNDS; SYNTHESIS; 080106* - Hydrogen- Production- Biosynthesis & Photochemical Processes; 010404 - Coal, Lignite, & Peat- Gasification; 550700 - Microbiology

Citation Formats

Clausen, E C, Gaddy, J L, and Ko, C W. Biological production of hydrogen. United States: N. p., 1992. Web.
Clausen, E C, Gaddy, J L, & Ko, C W. Biological production of hydrogen. United States.
Clausen, E C, Gaddy, J L, and Ko, C W. Wed . "Biological production of hydrogen". United States.
@article{osti_6928226,
title = {Biological production of hydrogen},
author = {Clausen, E C and Gaddy, J L and Ko, C W},
abstractNote = {All Phase I objectives were satisfactorily achieved. Stoichiometric yields of hydrogen were obtained from CO with R. rubrum. Fast growth was observed on synthesis gas only; however, growth was maximized with a small amount of yeast extract at 30[degrees]C and pH 7. Intrinsic kinetic parameters were determined and showed that fast hydrogen production rates are possible. The Phase II research program concentrated on defining the optimal culture and bioreactor for hydrogen production. To insure that the best possible system was developed, cultures were screened for enzyme activity and hydrogen production. Promising cultures were optimized for hydrogen yield and CO conversion. Bioreactors that achieved high mass transfer rates, as well as high cell concentrations, were studied. Advanced bioreactor concepts, such as solid-state fermentation, non-aqueous fermentation, and high pressure fermentation were applied to these reactors to enhance mass transport. Process design and economic evaluations of the various alternatives were used to guide the research program. A new bacterial culture has been isolated from natural sources which consumes CO rapidly and gives high hydrogen yields. The new isolate, ERIH2, is able to utilize a variety of sugars as growth substrates and, unlike R. rubrum, does not require light for growth. The yield of H[sub 2] by ERIH2 reach 100 percent. Typical H[sub 2] yields by R. rubrum were 75--85 percent.},
doi = {},
url = {https://www.osti.gov/biblio/6928226}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}

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