skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli

Abstract

Gamma irradiation of Escherichia coli B/r caused a dose-dependent inhibition of the capacity of the cells to synthesize L-arabinose isomerase in response to the inducer. At higher doses (18 krad and above), postirradiation incubation led to further inhibition of the capacity to synthesize L-arabinose isomerase, whereas cells receiving lower doses recovered from the damage to the enzyme synthesizing system following incubation. Cyclic AMP partially reversed the inhibitory effect on L-arabinose isomerase induction produced immediately after irradiation by all gamma-ray doses (up to 30 krad), but the enhanced inhibitory effect caused by induction in cells irradiated at higher doses could not be reversed by the nucleotide. It is suggested that although catabolite repression is partly responsible for causing the inhibition of the enzyme synthesizing capacity of the cells observed immediately after gamma irradiation, the enhanced inhibition caused by incubating cells irradiated at higher doses is not due to interference with the control mechanism regulated by catabolite repression.

Authors:
;
Publication Date:
Research Org.:
Banaras Hindu Univ., Varanasi, India
OSTI Identifier:
6928093
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; BIOLOGICAL RADIATION EFFECTS; ISOMERASES; ENZYME INDUCTION; ARABINOSE; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; ALDEHYDES; BACTERIA; BIOLOGICAL EFFECTS; CARBOHYDRATES; ELECTROMAGNETIC RADIATION; ENZYMES; GENE REGULATION; IONIZING RADIATIONS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; RADIATION EFFECTS; RADIATIONS; SACCHARIDES; 560130* - Radiation Effects on Microorganisms

Citation Formats

Chatterjee, A, and Bhattacharya, A K. Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli. United States: N. p., 1986. Web. doi:10.2307/3576824.
Chatterjee, A, & Bhattacharya, A K. Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli. United States. https://doi.org/10.2307/3576824
Chatterjee, A, and Bhattacharya, A K. 1986. "Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli". United States. https://doi.org/10.2307/3576824.
@article{osti_6928093,
title = {Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli},
author = {Chatterjee, A and Bhattacharya, A K},
abstractNote = {Gamma irradiation of Escherichia coli B/r caused a dose-dependent inhibition of the capacity of the cells to synthesize L-arabinose isomerase in response to the inducer. At higher doses (18 krad and above), postirradiation incubation led to further inhibition of the capacity to synthesize L-arabinose isomerase, whereas cells receiving lower doses recovered from the damage to the enzyme synthesizing system following incubation. Cyclic AMP partially reversed the inhibitory effect on L-arabinose isomerase induction produced immediately after irradiation by all gamma-ray doses (up to 30 krad), but the enhanced inhibitory effect caused by induction in cells irradiated at higher doses could not be reversed by the nucleotide. It is suggested that although catabolite repression is partly responsible for causing the inhibition of the enzyme synthesizing capacity of the cells observed immediately after gamma irradiation, the enhanced inhibition caused by incubating cells irradiated at higher doses is not due to interference with the control mechanism regulated by catabolite repression.},
doi = {10.2307/3576824},
url = {https://www.osti.gov/biblio/6928093}, journal = {Radiat. Res.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Sat Nov 01 00:00:00 EST 1986},
month = {Sat Nov 01 00:00:00 EST 1986}
}