Production of transforming growth factor. cap alpha. in human pancreatic cancer cells: evidence for a superagonist autocrine cycle
Previous work showed that cultured human pancreatic cancer cells overexpress the epidermal growth factor (EGF) receptor. In the present study, the authors sought to determine whether some of these cell lines produce transforming growth factor ..cap alpha.. (TGF-..cap alpha..). Utilizing a radiolabeled TGF-..cap alpha.. cDNA in hybridization experiments, they determined that ASPC-1, T/sub 3/M/sub 4/, PANC-1, COLO-357, and MIA PaCa-2 cell lines expressed TGF-..cap alpha.. mRNA. Serum-free medium conditioned by T/sub 3/M/sub 4/ and ASPC-1 cells contained significant amounts of TGF-..cap alpha.. protein. Although unlabeled TGF-..cap alpha.. readily competed with /sup 125/I-labeled EGF for binding, each cell line exhibited lower surface binding and internalization of /sup 125/I-labeled TGF-..cap alpha.. as compared to /sup 125/I-labeled EGF. Both TGF-..cap alpha.. and EGF significantly enhanced the anchorage-independent growth of PANC-1, T/sub 3/M/sub 4/, and ASPC-1 cells. However, TGF-..cap alpha.. was 10- to 100-fold more potent than EGF. These findings suggest that the concomitant overexpression of EGF receptors and production of TGF-..cap alpha.. may represent an efficient mechanism for certain cancer cells to obtain a growth advantage.
- Research Organization:
- Univ. of Arizona Health Sciences Center, Tucson (USA)
- OSTI ID:
- 6924088
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:21
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis and evaluation of a radioiodinated peptide probe targeting αvβ6 integrin for the detection of pancreatic ductal adenocarcinoma
Human transforming growth factor type. cap alpha. coding sequence is not a directed-acting oncogene when overexpressed in NIH 3T3 cells
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RADIOASSAY
RNA
AUTORADIOGRAPHY
HYBRIDIZATION
ELECTROPHORESIS
IODINE 125
MAN
PANCREAS
RECEPTORS
RECOMBINANT DNA
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550301 - Cytology- Tracer Techniques