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Title: Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines

Abstract

The authors investigated the role of homeobox-containing genes in human hematopoiesis because homeobox genes (i) control cell fate in the Drosophila embryo, (ii) are expressed in specific patterns in human embryos, and (iii) appear to function as transcription factors that control cell phenotype in other mammalian organs. Using four homeobox probes from the HOX2 locus and a previously undescribed homeobox cDNA (PL1), they screened mRNAs from 18 human leukemic cell lines representing erythroid, myeloid, and T- and B-cell lineages. Complex patterns of lineage-restricted expression are observed. No single homeobox gene is expressed in all types of hematopoietic cells, but each cell type exhibits homeobox gene expression. They have demonstrated (i) lineage-restricted expression of five homeobox genes in erythroid and monocytic cell lines; (ii) expression of additional homeobox genes in other cell lineages (HL-60 and lymphoid cells); (iii) expression of one homeobox gene in normal marrow cells; and (iv) modulation of expression during differentiation. These data suggest that these genes play a role in human hematopoietic development and lineage commitment.

Authors:
; ; ; ; ; ;  [1];  [2]
  1. Veterans Administration Medical Center, Martinez, CA (USA) Univ. of California, Davis (USA)
  2. Univ. of California, Berkeley (USA)
Publication Date:
OSTI Identifier:
6921527
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:21; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BONE MARROW CELLS; CELL DIFFERENTIATION; DNA SEQUENCING; GENE REGULATION; GENES; HEMATOPOIETIC SYSTEM; LEUKEMIA; MAN; MESSENGER-RNA; PHOSPHORUS 32; RNA-ASE; TUMOR CELLS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ENZYMES; ESTERASES; HEMIC DISEASES; HYDROLASES; IMMUNE SYSTEM DISEASES; ISOTOPES; LIGHT NUCLEI; MAMMALS; NEOPLASMS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; RNA; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Shen, W F, Largman, C, Lowney, P, Corral, J C, Detmer, K, Hack, F M, Lawrence, H J, and Hauser, C A. Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. United States: N. p., 1989. Web. doi:10.1073/pnas.86.21.8536.
Shen, W F, Largman, C, Lowney, P, Corral, J C, Detmer, K, Hack, F M, Lawrence, H J, & Hauser, C A. Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. United States. doi:10.1073/pnas.86.21.8536.
Shen, W F, Largman, C, Lowney, P, Corral, J C, Detmer, K, Hack, F M, Lawrence, H J, and Hauser, C A. Wed . "Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines". United States. doi:10.1073/pnas.86.21.8536.
@article{osti_6921527,
title = {Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines},
author = {Shen, W F and Largman, C and Lowney, P and Corral, J C and Detmer, K and Hack, F M and Lawrence, H J and Hauser, C A},
abstractNote = {The authors investigated the role of homeobox-containing genes in human hematopoiesis because homeobox genes (i) control cell fate in the Drosophila embryo, (ii) are expressed in specific patterns in human embryos, and (iii) appear to function as transcription factors that control cell phenotype in other mammalian organs. Using four homeobox probes from the HOX2 locus and a previously undescribed homeobox cDNA (PL1), they screened mRNAs from 18 human leukemic cell lines representing erythroid, myeloid, and T- and B-cell lineages. Complex patterns of lineage-restricted expression are observed. No single homeobox gene is expressed in all types of hematopoietic cells, but each cell type exhibits homeobox gene expression. They have demonstrated (i) lineage-restricted expression of five homeobox genes in erythroid and monocytic cell lines; (ii) expression of additional homeobox genes in other cell lineages (HL-60 and lymphoid cells); (iii) expression of one homeobox gene in normal marrow cells; and (iv) modulation of expression during differentiation. These data suggest that these genes play a role in human hematopoietic development and lineage commitment.},
doi = {10.1073/pnas.86.21.8536},
journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:21,
place = {United States},
year = {1989},
month = {11}
}