NMR study of the possible interaction in solution of angiotensin II with a peptide encoded by angiotensin II complementary RNA
- Abbott Labs., Abbott Park, IL (USA)
- Univ. of Texas, Austin (USA)
The potential binding of angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) (AII) to a peptide encoded by its complementary RNA (Lys-Gly-Val-Asp-Val-Try-Ala-Val) (IIA) has been studied by monitoring the {sup 1}H NMR spectrum of IIA in aqueous phosphate or Tris{center dot}HCl buffer ({sup 2}H{sub 2}O) as it is titrated with AII. For molar ratios of AII/IIA ranging from 0.2 to 1.8, the NMR spectra are unchanged as compared to the spectra of the isolated peptides. Based on these findings, the K{sub d} for the putative biomolecular complex of the two peptides under these conditions is calculated to be >10{sup {minus}4} M. This result does not support the suggestion of Elton et al. that AII and IIA engage in high-affinity binding (K{sub d} {approx} 5 {times} 10 {sup {minus}8} M) with each other.
- OSTI ID:
- 6921072
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:24; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANGIOTENSIN
NUCLEAR MAGNETIC RESONANCE
PEPTIDES
CROSS-LINKING
AQUEOUS SOLUTIONS
BIOCHEMICAL REACTION KINETICS
HEAVY WATER
MESSENGER-RNA
PROTONS
BARYONS
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
DISPERSIONS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
GLOBULINS
HADRONS
HYDROGEN COMPOUNDS
KINETICS
MAGNETIC RESONANCE
MIXTURES
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYMERIZATION
PROTEINS
REACTION KINETICS
RESONANCE
RNA
SOLUTIONS
VASOCONSTRICTORS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics