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Title: Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats

Abstract

To examine the pulmonary effects of relatively low levels of NO{sub 2} and O{sub 3}, and test for any possible interaction in their effects, we exposed 3-mo-old male Sprague-Dawley rats, free of specific pathogens, to either filtered room air (control) or 1.20 ppm (2256 micrograms/m3) NO{sub 2}, 0.30 ppm (588 micrograms/m3) O{sub 3}, or a combination of the two oxidants continuously for 3 d. We studied a series of parameters in the lung, including lung weight, and enzyme activities related to NADPH generation, sulfhydryl metabolism, and cellular detoxification. The results showed that relative to control, exposure to NO2 caused small but nonsignificant changes in all the parameters; O{sub 3} caused significant increases in all the parameters except for superoxide dismutase; and a combination of NO{sub 2} and O{sub 3} caused increases in all the parameters, and the increases were greater than those caused by NO{sub 2} or O{sub 3} alone. Statistical analysis of the data showed that the effects of combined exposure were synergistic for 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, glutathione reductase, and superoxide dismutase activities, and additive for glutathione peroxidase and disulfide reductase activities, but indifferent from those of O{sub 3} exposure for other enzyme activities.

Authors:
; ;  [1]
  1. Univ. of California, Los Angeles (USA)
Publication Date:
OSTI Identifier:
6919152
Resource Type:
Journal Article
Journal Name:
Journal of Toxicology and Environmental Health; (USA)
Additional Journal Information:
Journal Volume: 29:3; Journal ID: ISSN 0098-4108
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LUNGS; SENSITIVITY; NITROGEN DIOXIDE; TOXICITY; OZONE; DOSE-RESPONSE RELATIONSHIPS; ENZYME ACTIVITY; NADP; OXIDOREDUCTASES; RATS; REGRESSION ANALYSIS; SULFHYDRYL RADICALS; SUPEROXIDE DISMUTASE; SYNERGISM; ANIMALS; BODY; CHALCOGENIDES; COENZYMES; ENZYMES; MAMMALS; MATHEMATICS; NITROGEN COMPOUNDS; NITROGEN OXIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; RADICALS; RESPIRATORY SYSTEM; RODENTS; STATISTICS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Lee, J S, Mustafa, M G, and Afifi, A A. Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats. United States: N. p., 1990. Web. doi:10.1080/15287399009531392.
Lee, J S, Mustafa, M G, & Afifi, A A. Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats. United States. doi:10.1080/15287399009531392.
Lee, J S, Mustafa, M G, and Afifi, A A. Mon . "Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats". United States. doi:10.1080/15287399009531392.
@article{osti_6919152,
title = {Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats},
author = {Lee, J S and Mustafa, M G and Afifi, A A},
abstractNote = {To examine the pulmonary effects of relatively low levels of NO{sub 2} and O{sub 3}, and test for any possible interaction in their effects, we exposed 3-mo-old male Sprague-Dawley rats, free of specific pathogens, to either filtered room air (control) or 1.20 ppm (2256 micrograms/m3) NO{sub 2}, 0.30 ppm (588 micrograms/m3) O{sub 3}, or a combination of the two oxidants continuously for 3 d. We studied a series of parameters in the lung, including lung weight, and enzyme activities related to NADPH generation, sulfhydryl metabolism, and cellular detoxification. The results showed that relative to control, exposure to NO2 caused small but nonsignificant changes in all the parameters; O{sub 3} caused significant increases in all the parameters except for superoxide dismutase; and a combination of NO{sub 2} and O{sub 3} caused increases in all the parameters, and the increases were greater than those caused by NO{sub 2} or O{sub 3} alone. Statistical analysis of the data showed that the effects of combined exposure were synergistic for 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, glutathione reductase, and superoxide dismutase activities, and additive for glutathione peroxidase and disulfide reductase activities, but indifferent from those of O{sub 3} exposure for other enzyme activities.},
doi = {10.1080/15287399009531392},
journal = {Journal of Toxicology and Environmental Health; (USA)},
issn = {0098-4108},
number = ,
volume = 29:3,
place = {United States},
year = {1990},
month = {1}
}