Effects of severe hypercapnia on brain cell pH and high energy phosphate levels in conscious mice by /sup 31/P NMR
The authors have measured the effects of 10% CO/sub 2/ breathing (10% CO/sub 2/ in room air) on brain cell pH (pHi) and levels of phosphocreatine (PCr) and ATP in conscious mice (n=3) using /sup 31/P high resolution (8.4 tesla magnet) nuclear magnetic resonance spectroscopy (NMR) with surface coils. Brain cell pH decreased by 0.22 (+/- 0.02) pH units during the first 10 minutes of CO/sub 2/, but were restored to control values during the next 10 minutes. Washout of the CO/sub 2/, by substituting room air for the CO/sub 2/ mixture, resulted in a 0.15 (+/- 0.02) pH unit increase over controls within 10 minutes. The ratios of PCr/ATP remained unchanged throughout the experiment. They conclude that pHi regulation is rapid and complete during severe hypercapnia and that this compensation involves movement of ions rather than intracellular protein buffering since there is hysteresis in the compensatory process (pHi is alkaline during washout). Additionally, unlike previous reports using other techniques, they find no decrease in the levels of PCr with 10% CO/sub 2/ breathing.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6914038
- Report Number(s):
- CONF-8604222-; TRN: 87-006140
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BRAIN
PH VALUE
CARBON DIOXIDE
BIOLOGICAL EFFECTS
PHOSPHOCREATINE
TISSUE DISTRIBUTION
ATP
MICE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
AMINO ACIDS
ANIMALS
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHALCOGENIDES
DISTRIBUTION
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RESONANCE
RODENTS
SPECTRA
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
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550600 - Medicine