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Title: Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo

Abstract

Investigation of the biological N-methylation of amines has been conducted. Tryptamine and pyridine N-methylation reactions have been evaluated in vitro using rabbit lung and liver homogenates. The N-methylated derivatives of tryptamine and pyridine have been synthesized and used in the development of analytical systems, based upon high performance liquid chromatography. These analytical systems have allowed the unambiguous determination of the identity of the N-methylated derivatives of these amino compounds which are formed in tissue homogenates that have been incubated with S-adenosyl-L-(/sup 3/H-methyl)-methionine, thereby also establishing SAM as the co-factor in these enzymatic reactions. The in vivo metabolism of radiolabeled nicotine enantiomers to oxidative and N-methylated nicotine compounds has been studied. Analyses revealed that significant differences exist in the metabolism of radiolabeled nicotine enantiomers among the different animals species.

Authors:
Publication Date:
Research Org.:
Kentucky Univ., Lexington (USA)
OSTI Identifier:
6905558
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; METHYL TRANSFERASES; ENZYME ACTIVITY; NICOTINE; METABOLISM; METHYLATION; BIOLOGICAL PATHWAYS; GUINEA PIGS; HAMSTERS; HOMOGENATES; IN VIVO; LIQUID COLUMN CHROMATOGRAPHY; LIVER; LUNGS; METHIONINE; RABBITS; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALOIDS; AMINES; AMINO ACIDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZINES; AZOLES; BODY; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; DRUGS; ENZYMES; GLANDS; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PARASYMPATHOLYTICS; PARASYMPATHOMIMETICS; PYRIDINES; PYRROLES; PYRROLIDINES; RESPIRATORY SYSTEM; RODENTS; SEPARATION PROCESSES; TRANSFERASES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Nwosu, C G. Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo. United States: N. p., 1987. Web.
Nwosu, C G. Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo. United States.
Nwosu, C G. Thu . "Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo". United States.
@article{osti_6905558,
title = {Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo},
author = {Nwosu, C G},
abstractNote = {Investigation of the biological N-methylation of amines has been conducted. Tryptamine and pyridine N-methylation reactions have been evaluated in vitro using rabbit lung and liver homogenates. The N-methylated derivatives of tryptamine and pyridine have been synthesized and used in the development of analytical systems, based upon high performance liquid chromatography. These analytical systems have allowed the unambiguous determination of the identity of the N-methylated derivatives of these amino compounds which are formed in tissue homogenates that have been incubated with S-adenosyl-L-(/sup 3/H-methyl)-methionine, thereby also establishing SAM as the co-factor in these enzymatic reactions. The in vivo metabolism of radiolabeled nicotine enantiomers to oxidative and N-methylated nicotine compounds has been studied. Analyses revealed that significant differences exist in the metabolism of radiolabeled nicotine enantiomers among the different animals species.},
doi = {},
url = {https://www.osti.gov/biblio/6905558}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {1}
}

Thesis/Dissertation:
Other availability
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