Specific quantification of Trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies

Journal Article · Sat Oct 01 00:00:00 EDT 1994 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260440812· OSTI ID:6902730

Nidetzky, B ^[1]; Claeyssens, M ^[2]

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1. Univ. of Agriculture, Vienna (Austria). Inst. of Food Technology Technical Univ. of Graz (Austria). Inst. of Biotechnology
2. Univ. of Ghent (Belgium). Dept. of Biochemistry, Physiology, and Microbiology

Specific quantification of the major cellulolytic components of the Trichoderma reesei enzyme complex, i.e., endoglucanases I and III and cellobiohydrolases I and II, are described and, employing a defined mixture of these four cellulases reconstituted according to the composition of the native Trichoderma cellulase complex, used to determine the binding of each individual component onto filter paper. During substrate degradation by this enzyme mixture, the specific adsorption of each individual cellulase gradually increases and no preferential binding of one enzyme component in any particular phase of cellulose hydrolysis is found. T. reesei cellobiohydrolases I and II admixed with endoglucanases I and II represent a full-value'' cellulase system that is capable of degrading semicrystalline cellulose efficiently. In comparison with crude Trichoderma enzyme complex, almost identical adsorption properties and similar hydrolytic efficiency are found for the reconstituted mixture.

• OSTI ID: 6902730
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 44:8; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULASE
ENZYME ACTIVITY
CELLULOSE
ENZYMATIC HYDROLYSIS
TRICHODERMA
GROWTH
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
EUMYCOTA
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOLVOLYSIS
090900* - Biomass Fuels- Processing- (1990-)