Three-dimensional autoradiographic localization of quench-corrected glycine receptor specific activity in the mouse brain using sup 3 H-strychnine as the ligand

Journal Article · Thu Mar 01 00:00:00 EST 1990 · Journal of Neuroscience; (USA)

OSTI ID:6897217

White, W F; O'Gorman, S; Roe, A W ^[1]

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1. Harvard Medical School, Boston, MA (USA)

The autoradiographic analysis of neurotransmitter receptor distribution is a powerful technique that provides extensive information on the localization of neurotransmitter systems. Computer methodologies are described for the analysis of autoradiographic material which include quench correction, 3-dimensional display, and quantification based on anatomical boundaries determined from the tissue sections. These methodologies are applied to the problem of the distribution of glycine receptors measured by 3H-strychnine binding in the mouse CNS. The most distinctive feature of this distribution is its marked caudorostral gradient. The highest densities of binding sites within this gradient were seen in somatic motor and sensory areas; high densities of binding were seen in branchial efferent and special sensory areas. Moderate levels were seen in nuclei related to visceral function. Densities within the reticular formation paralleled the overall gradient with high to moderate levels of binding. The colliculi had low and the diencephalon had very low levels of binding. No binding was seen in the cerebellum or the telencephalon with the exception of the amygdala, which had very low levels of specific binding. This distribution of glycine receptors correlates well with the known functional distribution of glycine synaptic function. These data are illustrated in 3 dimensions and discussed in terms of the significance of the analysis techniques on this type of data as well as the functional significance of the distribution of glycine receptors.

• OSTI ID: 6897217
• Journal Information: Journal of Neuroscience; (USA), Vol. 10:3; ISSN 0270-6474
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
GLYCINE
RECEPTORS
BIOLOGICAL LOCALIZATION
BIOCHEMICAL REACTION KINETICS
BIOELECTRICITY
LIGANDS
MICE
STRYCHNINE
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
ALKALOIDS
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
AZOLES
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
ELECTRICITY
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INDOLES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques