Metal ion induced phase changes in lipid vesicles: Building a membrane assisted receptor/transducer system

Conference · Wed Jan 01 00:00:00 EST 1992 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6894159

Singh, A; Tsao, L I; Gaber, B P ^[1]

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1. Center for BioMolecular Science and Engineering, Washington, DC (United States) Georgetown Univ., Washington, DC (United States)

The potential of metal binding amphiphiles in bilayer assemblies as components for metal ion detection is studied. Single chain amphiphiles equipped with an azobenzene chromophore in their alkyl chains and a cyclic tetramine, cyclam in the headgroup region are synthesized. In these compounds cyclam functions as metal binding site and azobenzene optically reports the binding event. The aggregation behavior of free and metal-bound cyclam amphiphiles incorporated in dihexadecyl dimethyl ammonium bromide (CN-4) bilayers in studied spectrophotometrically. The cyclam azobenzene amphiphiles aggregate in the CN-4 bilayers below the chain melting temperature of CH-4 to produce a hypsochromic shift. Deaggregation occurs above 27.3 C as evidenced by re-emergence of the 356 nm peak. Metal ion binding stabilizes the aggregates up to 45 C, above which an absorption maxima at 356 nm is observed. Thus, at 30 C a linear decrease in an absorption at 356 nm was observed in the presence of the metal ions. The relevance of spectral shift due to metal-cyclam interaction in the development of an efficient metal ion detector will be discussed.

• OSTI ID: 6894159
• Report Number(s): CONF-9202109-; CODEN: FAJOEC
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 6:1; Conference: American Society for Biochemistry and Molecular Biology and Biophysical Society joint meeting, Houston, TX (United States), 9-13 Feb 1992; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LIPIDS
MOLECULAR STRUCTURE
METALS
BIOLOGICAL EFFECTS
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
RECEPTORS
CELL CONSTITUENTS
ELEMENTS
KINETICS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
560300* - Chemicals Metabolism & Toxicology