skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch

Abstract

In the mouse pre-B-cell line 18-81, cells can switch production in vitro from immunoglobulin ..mu.. chain to ..gamma..2b chain. The gene encoding the ..gamma..2b chain is created by a rearrangement of the ..mu.. gene. This rearrangement always takes place within a homolog. In cells with a ..gamma..2b gene, most of the time the gene segment encoding the constant region of the ..mu.. chain is deleted, but often the rearrangement leads to cells that produce no immunoglobulin, and all DNA sequences are retained. The latter result is due to an inversion. Inversions exclude the unequal sister chromatid exchange model of the heavy-chain class switch. Looping out is an intermediate step in the process of generating an inversion. These finding demonstrate that the switch rearrangement occurs by looping out and deletion.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of California, San Francisco (USA)
OSTI Identifier:
6879720
DOE Contract Number:  
AC03-76SF01012
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 85:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMOSOMAL ABERRATIONS; MOLECULAR BIOLOGY; IMMUNOGLOBULINS; BIOSYNTHESIS; DNA SEQUENCING; ELECTROPHORESIS; IN VITRO; LYMPHOCYTES; METHIONINE; MICE; SISTER CHROMATID EXCHANGES; SULFUR 35; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; GLOBULINS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; MATERIALS; MUTATIONS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; SULFUR ISOTOPES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Jaeck, H M, McDowell, M, Steinberg, C M, and Wabl, M. Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch. United States: N. p., 1988. Web. doi:10.1073/pnas.85.5.1581.
Jaeck, H M, McDowell, M, Steinberg, C M, & Wabl, M. Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch. United States. https://doi.org/10.1073/pnas.85.5.1581
Jaeck, H M, McDowell, M, Steinberg, C M, and Wabl, M. 1988. "Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch". United States. https://doi.org/10.1073/pnas.85.5.1581.
@article{osti_6879720,
title = {Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch},
author = {Jaeck, H M and McDowell, M and Steinberg, C M and Wabl, M},
abstractNote = {In the mouse pre-B-cell line 18-81, cells can switch production in vitro from immunoglobulin ..mu.. chain to ..gamma..2b chain. The gene encoding the ..gamma..2b chain is created by a rearrangement of the ..mu.. gene. This rearrangement always takes place within a homolog. In cells with a ..gamma..2b gene, most of the time the gene segment encoding the constant region of the ..mu.. chain is deleted, but often the rearrangement leads to cells that produce no immunoglobulin, and all DNA sequences are retained. The latter result is due to an inversion. Inversions exclude the unequal sister chromatid exchange model of the heavy-chain class switch. Looping out is an intermediate step in the process of generating an inversion. These finding demonstrate that the switch rearrangement occurs by looping out and deletion.},
doi = {10.1073/pnas.85.5.1581},
url = {https://www.osti.gov/biblio/6879720}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:5,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 1988},
month = {Tue Mar 01 00:00:00 EST 1988}
}